Use of the 16S–23S ribosomal genes spacer region for the molecular typing of sphingomonads

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dc.contributor.author Tokajian, Sima
dc.contributor.author Hashwa, Fuad
dc.contributor.author El-Medawar, Siba
dc.date.accessioned 2016-03-23T09:18:16Z
dc.date.available 2016-03-23T09:18:16Z
dc.date.copyright 2008
dc.date.issued 2016-03-23
dc.identifier.issn 0008-4166 en_US
dc.identifier.uri http://hdl.handle.net/10725/3390
dc.description.abstract The ability of sphingomonads in drinking water to cause community- and hospital-acquired opportunistic infections has raised the need to establish reproducible identification assays. In this study, a total of 129 isolates recovered from drinking water with yellow- to orange-pigmented colonies were distributed among 10 biotypes on the basis of colony morphology. Polymorphisms, based on the amplification and restriction digestion of the intergenic transcribed spacer (ITS) region within the 10 assigned biotypes and 18 ATCC reference strains, were used to investigate the ability of this approach to differentiate closely related sphingomonads. ITS size, which ranged between 400 and 1100 bp, did not vary enough among the different genera. However, 16 distinct banding patterns within the ATCC reference strains and 9 within the 10 biotypes were obtained through ITS restriction digestion, and the majority of the tested biotypes produced patterns similar to those generated by the ATCC strains. To our knowledge, this study is not only the first comprehensive record of the size of the ITS region in sphingomonads, it is also the first study that describes the use of ITS restriction digestion to subtype those isolates. en_US
dc.language.iso en en_US
dc.title Use of the 16S–23S ribosomal genes spacer region for the molecular typing of sphingomonads en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 199736770 en_US
dc.author.idnumber 199390070 en_US
dc.author.woa N/A en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Canadian Journal of Microbiology en_US
dc.journal.volume 54 en_US
dc.journal.issue 8 en_US
dc.article.pages 668-676 en_US
dc.keywords Sphingomonads en_US
dc.keywords ITS en_US
dc.keywords 16S rDNA en_US
dc.keywords Fingerprinting en_US
dc.identifier.doi http://dx.doi.org/10.1139/W08-054 en_US
dc.identifier.ctation Tokajian, S., Al-Medawar, S., & Hashwa, F. (2008). Use of the 16S-23S ribosomal genes spacer region for the molecular typing of sphingomonads. Canadian journal of microbiology, 54(8), 668-676. en_US
dc.author.email stokjian@lau.edu.lb
dc.author.email fhashwa@lau.edu.lb
dc.identifier.url http://www.nrcresearchpress.com/doi/abs/10.1139/W08-054#.V6MQG7h97y0

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