Phylogenetic assessment of heterotrophic bacteria from a water distribution system using 16S rDNA sequencing

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dc.contributor.author Tokajian, Sima T.
dc.contributor.author Hashwa, Fuad A.
dc.contributor.author Zalloua, Pierre A.
dc.contributor.author Hancock, Ian C.
dc.date.accessioned 2016-03-23T09:03:23Z
dc.date.available 2016-03-23T09:03:23Z
dc.date.copyright 2005
dc.date.issued 2016-03-23
dc.identifier.issn 0008-4166 en_US
dc.identifier.uri http://hdl.handle.net/10725/3389
dc.description.abstract Determination of a heterotrophic plate count (HPC) for drinking-water samples alone is not enough to assess possible health hazards associated with sudden changes in the bacterial count. Speciation is very crucial to determine whether the population includes pathogens and (or) opportunistic pathogens. Most of the isolates recovered from drinking water samples could not be allocated to a specific phylogenetic branch based on the use of conventional diagnostic methods. The present study had to use phylogenetic analysis, which was simplified by determining and using the first 500-bp sequence of the 16S rDNA, to successfully identify the type and species of bacteria found in the samples. Gram-positive bacteria α-, β-, and γ-Proteobacteria were found to be the major groups representing the heterotrophic bacteria in drinking water. The study also revealed that the presence of sphingomonads in drinking water supplies may be much more common than has been reported so far and thus further studies are merited. The intermittent mode of supply, mainly characterized by water stagnation and flow interruption associated possibly with biofilm detachment, raised the possibility that the studied bacterial populations in such systems represented organisms coming from 2 different niches, the biofilm and the water column.Key words: intermittent water supply, 16S rDNA, biofilm, water column. en_US
dc.language.iso en en_US
dc.title Phylogenetic assessment of heterotrophic bacteria from a water distribution system using 16S rDNA sequencing en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 199390070 en_US
dc.author.idnumber 199736770 en_US
dc.author.idnumber 200300001 en_US
dc.author.woa N/A en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Canadian Journal of Microbiology en_US
dc.journal.volume 51 en_US
dc.journal.issue 4 en_US
dc.article.pages 325-335 en_US
dc.identifier.doi http://dx.doi.org/10.1139/w05-007 en_US
dc.identifier.ctation Tokajian, S. T., Hashwa, F. A., Hancock, I. C., & Zalloua, P. A. (2005). Phylogenetic assessment of heterotrophic bacteria from a water distribution system using 16S rDNA sequencing. Canadian journal of microbiology, 51(4), 325-335. en_US
dc.author.email stokjian@lau.edu.lb
dc.author.email fhashwa@lau.edu.lb
dc.author.email pierre.zalloua@lau.edu.lb
dc.identifier.url http://www.nrcresearchpress.com/doi/abs/10.1139/w05-007#.V6GoU7h97cs
dc.orcid.id https://orcid.org/0000-0002-8494-5081

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