Microsatellite DNA Identification and Genotyping of Candida albicans from Lebanese Clinical Isolates

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dc.contributor.author Khalaf, Roy A.
dc.contributor.author Barada, Ghyda
dc.contributor.author Basma, Raida
dc.date.accessioned 2016-03-21T08:00:14Z
dc.date.available 2016-03-21T08:00:14Z
dc.date.copyright 2008
dc.date.issued 2016-03-21
dc.identifier.issn 0301-486X en_US
dc.identifier.uri http://hdl.handle.net/10725/3367
dc.description.abstract The present study involves collecting 125 isolates labeled as C. albicans from five different Lebanese hospitals and utilizing the microsatellite genotyping test to determine the following: first, the accuracy of hospital identification by comparing microsatellite results to hospital results. Second, the frequency and genotypes of infectious strains present relative to tissue and hospital location- a possible indicator of nosocomial infection, and third, a possible relationship between lack of microsatellite heterozygosity to azole resistance. Our results showed that the error in hospital identification varied from 2 to 33%, averaging at 7%, with the highest identification error in stool. Misidentified isolates were mainly Candida tropicalis followed by C. glabrata and C. parapsilosis. Strains with similar genotypes were also found to occur within certain hospitals suggesting the possibility of nosocomial infection. Finally, a relationship between lack of heterozygosity and azole resistance was observed since nine out of 10 homozygous isolates sharing a common allele with a heterozygote strain were sensitive to all drugs tested, whereas the homozygous genotype was resistant to at least one drug. en_US
dc.language.iso en en_US
dc.title Microsatellite DNA Identification and Genotyping of Candida albicans from Lebanese Clinical Isolates en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 200300427 en_US
dc.author.woa N/A en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Mycopathologia en_US
dc.journal.volume 165 en_US
dc.article.pages 115-125 en_US
dc.keywords Candida albicans en_US
dc.keywords Microsatellite genotyping en_US
dc.keywords Nosocomial infection en_US
dc.keywords Drug resistance en_US
dc.identifier.doi http://dx.doi.org/10.1007/s11046-008-9089-0 en_US
dc.identifier.ctation Barada, G., Basma, R., & Khalaf, R. A. (2008). Microsatellite DNA identification and genotyping of Candida albicans from Lebanese clinical isolates. Mycopathologia, 165(3), 115-125. en_US
dc.author.email roy.khalaf@lau.edu.lb
dc.identifier.url http://link.springer.com/article/10.1007/s11046-008-9089-0

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