Comparative proteomic analysis of a Candida albicans DSE1 mutant under filamentous and non-filamentous conditions

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dc.contributor.author Khalaf, Roy A.
dc.contributor.author Wex, Brigitte
dc.contributor.author Zohbi, Rasha
dc.date.accessioned 2016-03-21T07:43:59Z
dc.date.available 2016-03-21T07:43:59Z
dc.date.copyright 2014
dc.date.issued 2016-03-21
dc.identifier.issn 0749-503X en_US
dc.identifier.uri http://hdl.handle.net/10725/3366
dc.description.abstract Candida albicans is a common opportunistic pathogen that causes a variety of diseases in immunocompromised hosts. In a pathogen, cell wall proteins are important virulence factors. We previously characterized Dse1 as a cell wall protein necessary for virulence and resistance to cell surface-disrupting agents, such as Calcofluor white, chitin deposition, proper adhesion and biofilm formation. In the absence of decomplexation, our objectives were to investigate differential proteomic expression of a DSE1 mutant strain compared to the wild-type strain. The strains were grown under filamentous and non-filamentous conditions. The extracted cell proteome was subjected to tryptic digest, followed by generation of peptide profiles using MALDI–TOF MS. Generated peptide profiles were analysed and unique peaks for each strain and growth condition mined against a Candida database, allowing protein identification. The DSE1 mutant was shown to lack the chitin biosynthesis protein Chs5, explaining the previously observed decrease in chitin biosynthesis. The wild-type strain expressed Pra1, involved in pH response and zinc acquisition, Atg15, a lipase involved in virulence, and Sod1, required for oxidative stress tolerance, in addition to proteins involved in protein biosynthesis, explaining the increase in total protein content observed compared to the mutants strain. The mutant, on the other hand, expressed glucoamylase 1, a cell wall glycoprotein involved in carbohydrate metabolism cell wall degradation and biofilm formation. As such, MALDI–TOF MS is a reliable technique in identifying mutant-specific protein expression in C. albicans. Copyright © 2014 John Wiley & Sons, Ltd. en_US
dc.language.iso en en_US
dc.title Comparative proteomic analysis of a Candida albicans DSE1 mutant under filamentous and non-filamentous conditions en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 200300427 en_US
dc.author.woa N/A en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Yeast en_US
dc.journal.volume 31 en_US
dc.journal.issue 11 en_US
dc.article.pages 441-448 en_US
dc.keywords C. albicans en_US
dc.keywords DSE1 en_US
dc.keywords Chitin en_US
dc.keywords MALDI–TOF MS en_US
dc.keywords Peptide mass fingerprinting of peptide mixture en_US
dc.identifier.doi http://dx.doi.org/10.1002/yea.3039 en_US
dc.identifier.ctation Zohbi, R., Wex, B., & Khalaf, R. A. (2014). Comparative proteomic analysis of a Candida albicans DSE1 mutant under filamentous and non‐filamentous conditions. Yeast, 31(11), 441-448. en_US
dc.author.email roy.khalaf@lau.edu.lb
dc.identifier.url http://onlinelibrary.wiley.com/doi/10.1002/yea.3039/full

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