Nitrogen metabolism and virulence of Candida albicans require the GATA-type transcriptional activator encoded by GAT1

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dc.contributor.author Khalaf, Roy A.
dc.contributor.author Limjindaporn, Thawornchai
dc.contributor.author Fonzi, William A.
dc.date.accessioned 2016-03-18T07:55:35Z
dc.date.available 2016-03-18T07:55:35Z
dc.date.copyright 2003
dc.date.issued 2016-03-18
dc.identifier.issn 0950-382X en_US
dc.identifier.uri http://hdl.handle.net/10725/3359
dc.description.abstract Nitrogen acquisition and metabolism is central to microbial growth. A conserved family of zinc-finger containing transcriptional regulators known as GATA-factors ensures efficient utilization of available nitrogen sources by fungi. GATA factors activate expression of nitrogen catabolic pathways when preferred nitrogen sources are absent or limiting, a phenomenon known as nitrogen catabolite repression. GAT1 of Candida albicans encodes a GATA-factor homologous to the AREA protein of Aspergillus nidulans and related transcription factors involved in nitrogen regulation. Two observations implicated GAT1 in nitrogen regulation. The growth of mutants lacking GAT1 was reduced when isoleucine, tyrosine or tryptophan were the sole source of nitrogen. Secondly, when cultured on a secondary nitrogen source, gat1Δ mutants were unable to activate expression of GAP1, UGA4 or DAL5, which were shown to be nitrogen regulated in C. albicans. This regulatory defect did not prevent filamentation of gat1Δ mutants in nitrogen repressing or non-repressing conditions, demonstrating that nitrogen catabolite repression does not influence dimorphism. The mutants were, however, highly attenuated in a murine model of disseminated candidiasis. Attenuation was not associated with any diminution of growth in serum or ability to utilize serum amino acids. The results indicate an important role for nitrogen regulation in the virulence of C. albicans. en_US
dc.language.iso en en_US
dc.title Nitrogen metabolism and virulence of Candida albicans require the GATA-type transcriptional activator encoded by GAT1 en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 200300427 en_US
dc.author.woa N/A en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Molecular microbiology en_US
dc.journal.volume 50 en_US
dc.journal.issue 3 en_US
dc.article.pages 993-1004 en_US
dc.identifier.doi http://dx.doi.org/10.1046/j.1365-2958.2003.03747.x en_US
dc.identifier.ctation Limjindaporn, T., Khalaf, R. A., & Fonzi, W. A. (2003). Nitrogen metabolism and virulence of Candida albicans require the GATA‐type transcriptional activator encoded by GAT1. Molecular microbiology, 50(3), 993-1004. en_US
dc.author.email roy.khalaf@lau.edu.lb
dc.identifier.url http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2958.2003.03747.x/full

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