The DNA Binding Protein Rfg1 Is a Repressor of Filamentation in Candida albicans

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dc.contributor.author Khalaf, Roy A.
dc.contributor.author Zitomer, Richard S.
dc.date.accessioned 2016-03-18T07:36:38Z
dc.date.available 2016-03-18T07:36:38Z
dc.date.copyright 2001
dc.date.issued 2016-03-18
dc.identifier.issn 0016-6731 en_US
dc.identifier.uri http://hdl.handle.net/10725/3358
dc.description.abstract We have identified a repressor of hyphal growth in the pathogenic yeast Candida albicans. The gene was originally cloned in an attempt to characterize the homologue of the Saccharomyces cerevisiae Rox1, a repressor of hypoxic genes. Rox1 is an HMG-domain, DNA binding protein with a repression domain that recruits the Tup1/Ssn6 general repression complex to achieve repression. The C. albicans clone also encoded an HMG protein that was capable of repression of a hypoxic gene in a S. cerevisiae rox1 deletion strain. Gel retardation experiments using the purified HMG domain of this protein demonstrated that it was capable of binding specifically to a S. cerevisiae hypoxic operator DNA sequence. These data seemed to indicate that this gene encoded a hypoxic repressor. However, surprisingly, when a homozygous deletion was generated in C. albicans, the cells became constitutive for hyphal growth. This phenotype was rescued by the reintroduction of the wild-type gene on a plasmid, proving that the hyphal growth phenotype was due to the deletion and not a secondary mutation. Furthermore, oxygen repression of the hypoxic HEM13 gene was not affected by the deletion nor was this putative ROX1 gene regulated positively by oxygen as is the case for the S. cerevisiae gene. All these data indicate that this gene, now designated RFG1 for Repressor of Filamentous Growth, is a repressor of genes required for hyphal growth and not a hypoxic repressor. en_US
dc.language.iso en en_US
dc.title The DNA Binding Protein Rfg1 Is a Repressor of Filamentation in Candida albicans en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 200300427 en_US
dc.author.woa N/A en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Genetics en_US
dc.journal.volume 157 en_US
dc.journal.issue 4 en_US
dc.article.pages 1503-1512 en_US
dc.identifier.ctation Khalaf, R. A., & Zitomer, R. S. (2001). The DNA binding protein Rfg1 is a repressor of filamentation in Candida albicans. Genetics, 157(4), 1503-1512. en_US
dc.author.email roy.khalaf@lau.edu.lb
dc.identifier.url http://www.genetics.org/content/157/4/1503.short

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