The mechanism of action of kefir and its effect on the motility of breast and colorectal cancer cells. (c2013)

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dc.contributor.author Khoury, Nathalie El
dc.date.accessioned 2016-03-07T09:09:57Z
dc.date.available 2016-03-07T09:09:57Z
dc.date.copyright 5/28/2013 en_US
dc.date.issued 2016-03-07
dc.identifier.uri http://hdl.handle.net/10725/3288
dc.description.abstract Kefir which is a fermented milk product originating from the Caucasus Mountains is gaining increasing popularity due to its attribution in preventing or curing many chronic diseases among them cancer. Previous studies have focused on the anti-tumoral activity of kefir in vivo yet not extensive studies have been performed to elucidate its mechanism of action. Till now kefir is known to modulate intestinal microbiota, modulate the activity of immune system cells, and suspected to induce apoptosis in cancer cells in vivo. Recent studies have focused on the direct effect of kefir cell-free fractions on cancer cells in vitro. Earlier work in our lab has shown that cell-free fractions of kefir have anti-proliferative and pro-apoptotic effect on Caco-2 and HT-29 colorectal adenocarcinoma cells. In this study, we aim to determine a possible mechanism of action of kefir and to study its effect on the motility of colorectal and breast cancer cell lines. Results from the reverse-transcription PCR experiment have shown that kefir decreases the expression of TGF-α and TGF-β1 in HT-29 cells in a dose dependent manner. An increase in the production of both cytokines is usually associated with neoplastic transformation. Western blot assay results confirmed the previously observed increase in apoptosis as it showed an increase in Bax:Bcl-2 expression upon kefir treatment. Also an increase in p53 independent-p21 expression was observed in HT-29 cells upon kefir treatment which would explain the anti-proliferative effect of kefir. Furthermore, results from the time-lapse movies, wound-healing, and invasion assays showed no effect on the motility of colorectal (Caco-2 and HT-29) as well as breast (MCF-7 and MB-MDA-231) cancer cells upon kefir treatment. en_US
dc.language.iso en en_US
dc.subject Kefir -- Health aspects en_US
dc.subject Kefir -- Therapeutic use en_US
dc.subject Colon (Anatomy) -- Cancer -- Treatment en_US
dc.subject Rectum -- Cancer -- Treatment en_US
dc.subject Breast -- Cancer -- Treatment en_US
dc.subject Lebanese American University -- Dissertations en_US
dc.subject Dissertations, Academic en_US
dc.title The mechanism of action of kefir and its effect on the motility of breast and colorectal cancer cells. (c2013) en_US
dc.type Thesis en_US
dc.term.submitted Spring en_US
dc.author.degree MS in Molecular Biology en_US
dc.author.school SAS en_US
dc.author.idnumber 201005156 en_US
dc.author.commembers Wex, Brigitte
dc.author.commembers Mroueh, Mohammad
dc.author.woa OA en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.description.physdesc 1 hard copy: xiv, 39 leaves; ill.; 30 cm. available at RNL. en_US
dc.author.advisor Rizk, Sandra
dc.author.advisor El-Sibai, Mirvat
dc.keywords Colorectal Cancer en_US
dc.keywords Breast Cancer en_US
dc.keywords Kefir en_US
dc.keywords Proliferation en_US
dc.keywords Apoptosis en_US
dc.keywords Cell Motility en_US
dc.keywords Cell Invasion en_US
dc.description.bibliographiccitations Includes bibliographical references (leaves 31-39). en_US
dc.identifier.doi https://doi.org/10.26756/th.2013.53 en_US
dc.publisher.institution Lebanese American University en_US

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