Towards S. aureus maldi-typing. (c2014)

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dc.contributor.author Awad, Dania Bassam
dc.date.accessioned 2016-03-03T13:52:31Z
dc.date.available 2016-03-03T13:52:31Z
dc.date.copyright 4/25/2014 en_US
dc.date.issued 2016-03-03
dc.identifier.uri http://hdl.handle.net/10725/3260
dc.description.abstract Staphylococcus aureus is possibly the most common human pathogen causing approximately one third of all bacteria-related diseases. S. aureus’ adaptable characteristics and increasing antibiotic resistance allows it to thrive in both nosocomial and community settings. Identification of S. aureus is mainly performed via phenotypic methods and costly genotypic methods. Recently, Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) has been introduced into clinical microbiology as a means of bacterial identification to the species level. In this study, we compared MALDI protein fingerprints to genomic data of 25 S. aureus isolates, an S. epidermidis strain and an E. coli strain finding only geographic separation as visualized in a dendrogram. The absence of correlation between genomic and proteomic data to the strain level is owed to genomic mutations leading to protein isomers being falsely identified as different proteins at the MALDI level. Tryptic digests of protein extracts from one isolate were further characterized by NanoFlow Liquid Chromatography coupled with MALDI-TOF MS/MS and 94 proteins were successfully identified. Three candidate proteins are proposed for MALDI-TOF MS typing, which marks a step towards fast identification of S. aureus to the strain level via MALDI-TOF MS. en_US
dc.language.iso en en_US
dc.subject Proteomics en_US
dc.subject Staphylococcus aureus en_US
dc.subject Matrix-assisted laser desorption-ionization en_US
dc.subject Proteins -- Analysis en_US
dc.subject Lebanese American University -- Dissertations en_US
dc.subject Dissertations, Academic en_US
dc.title Towards S. aureus maldi-typing. (c2014) en_US
dc.type Thesis en_US
dc.title.subtitle genotypic correlation and shotgun proteomic identification en_US
dc.term.submitted Spring en_US
dc.author.degree MS in Molecular Biology en_US
dc.author.school SAS en_US
dc.author.idnumber 200902633 en_US
dc.author.commembers Tokajian, Sima
dc.author.commembers Khalaf, Roy
dc.author.woa OA en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.description.physdesc 1 hard copy: xvi, 71 leaves; ill.; 30 cm. available at RNL. en_US
dc.author.advisor Wex, Brigitte
dc.keywords Proteomics en_US
dc.keywords Staphylococcus aureus en_US
dc.keywords Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) en_US
dc.keywords NanoFlow Liquid Chromatography en_US
dc.keywords Typing en_US
dc.description.bibliographiccitations Includes bibliographical references (leaves 56-60). en_US
dc.identifier.doi https://doi.org/10.26756/th.2014.64 en_US
dc.publisher.institution Lebanese American University en_US

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