RHO A versus RHO C. (c2014)

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dc.contributor.author Dimassi, Saleh B. Al
dc.date.accessioned 2016-03-03T13:02:46Z
dc.date.available 2016-03-03T13:02:46Z
dc.date.copyright 5/26/2014 en_US
dc.date.issued 2016-03-03
dc.identifier.uri http://hdl.handle.net/10725/3257
dc.description.abstract Malignant astrocytomas are associated with high mortality rates; these tumors are highly invasive into adjacent areas of the normal brain. It has been established that Rho family of GTPases play a central role in regulation of cell migration and invasion. The Rho family is ras-related, consisting of the Rho, Rac, and CDC42 subfamilies. The Rho Protein has subtypes designated as RhoA, RhoB, RhoC, RhoD, RhoE, and RhoH genes. In this study we aim to look at the role both Rho A and Rho C play in protrusion formation where we found that upon RhoA knockdown protrusions are no longer formed, using 2D time lapse microscopy that shows the depletion of RhoA and RhoC leads to the decrease in 2D motility, RhoA knockdown decreases adhesion of cells in contrast RhoC knockdown increases adhesion of astrocytoma, and FRET based biosensors. In addition to study the role both proteins play in invadopodia formation which is not RhoA dependent according to our data, and cellular invasion where our results shows an increase in cell invasion upon both RhoA and RhoC knockdown. This study examines for the first time, the differential roles of RhoA and RhoC isoforms in astrocytoma motility and invasion. en_US
dc.language.iso en en_US
dc.subject Rho GTPases en_US
dc.subject Brain -- Cancer en_US
dc.subject Cells -- Motility en_US
dc.subject Astrocytomas -- Diagnosis en_US
dc.subject Lebanese American University -- Dissertations en_US
dc.subject Dissertations, Academic en_US
dc.title RHO A versus RHO C. (c2014) en_US
dc.type Thesis en_US
dc.title.subtitle role in invadopodia formation and invasion of astrocytoma cells en_US
dc.term.submitted Spring en_US
dc.author.degree MS in Molecular Biology en_US
dc.author.school SAS en_US
dc.author.idnumber 201100372 en_US
dc.author.commembers Abi Habib, Ralph
dc.author.commembers Rizk-Jamati, Sandra
dc.author.woa OA en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.description.physdesc 1 hard copy: xiii, 50 leaves, ill.; 30 cm. available at RNL. en_US
dc.author.advisor El Sibai, Mirvat
dc.keywords RhoA en_US
dc.keywords RhoC en_US
dc.keywords Invadopodia en_US
dc.keywords Invasion en_US
dc.keywords Fret en_US
dc.keywords Motility en_US
dc.description.bibliographiccitations Includes bibliographical references (leaves 43-50). en_US
dc.identifier.doi https://doi.org/10.26756/th.2014.61 en_US
dc.publisher.institution Lebanese American University en_US

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