Roles of RhoA, RhoC and RhoG in Secretion of Vascular Endothelial Growth Factor (VEGF) by Astrocytoma Cells and Angiogenesis in Vascular Endothelial Cells. (c2015)

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dc.contributor.author Fakih, Amira K.
dc.date.accessioned 2016-02-17T11:56:33Z
dc.date.available 2016-02-17T11:56:33Z
dc.date.issued 2016-02-17
dc.date.submitted 2015-04-27
dc.identifier.uri http://hdl.handle.net/10725/3112
dc.description.abstract As the tumor size increases, cancer cells stimulate neighboring vascular endothelial cells to proliferate and form new blood vessels in order to irrigate the tumor. This is through the secretion of vascular endothelial growth factors (VEGF) by the cancer cells that stimulate angiogenesis in vascular endothelial cells. The Rho GTPase family of proteins regulates VEGF secretion by cancer cells and regulates angiogenesis in vascular endothelial cells. Our study aims to elucidate the role of different isoforms of Rho GTPases in Astrocytoma cell expression of VEGF and blood vessels formation in vascular endothelial cells. The role of Rho GTPases in angiogenesis signaling was first examined in vascular endothelial cells. In ECV cells, the knock down of RhoA, RhoC and RhoG reduced the blood tubes formation. Knocking down StarD13, a RhoA and Cdc42 GAP, led to increase in angiogenesis and blood vessel formation. Second, the role of RhoA and RhoC on VEGF levels of expression on Astrocytoma cells was studied; results showed that RhoA regulated VEGF expression more efficiently than RhoC. The role of the PI3K pathway and VEGF secretion in SF268 Astrocytoma cells in response to EGF stimulation or in response to starvation was examined. The study showed that as the cancer cells are deprived from their physiological conditions for a longer period of time as they express more VEGF, MMP-2 and MMP-9. In addition, PI3K pathway is activated after EGF stimulation in tumor cells. In conclusion, this study will explicate the cross-talk between Astrocytoma cells and vascular endothelial cells and the pathways responsible for angiogenesis development en_US
dc.language.iso en en_US
dc.subject Vascular endothelial growth factors en_US
dc.subject Astrocytomas en_US
dc.subject Rho GTPases en_US
dc.subject Tumors -- Blood-vessels -- Growth en_US
dc.subject Lebanese American University -- Dissertations en_US
dc.subject Dissertations, Academic en_US
dc.title Roles of RhoA, RhoC and RhoG in Secretion of Vascular Endothelial Growth Factor (VEGF) by Astrocytoma Cells and Angiogenesis in Vascular Endothelial Cells. (c2015) en_US
dc.type Thesis en_US
dc.term.submitted Spring en_US
dc.author.degree MS in Molecular Biology en_US
dc.author.school SAS en_US
dc.author.idnumber 200700998 en_US
dc.author.commembers Abi Habib, Ralph
dc.author.commembers Rizk, Sandra
dc.author.woa OA en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.description.physdesc 1 hard copy: xiv, 62 leaves; ill. (some col.); 30 cm. available at RNL. en_US
dc.author.advisor El-Sibai, Mirvat
dc.keywords Angiogenesis en_US
dc.keywords Rho-GTPases en_US
dc.keywords PI3K en_US
dc.keywords VEGF en_US
dc.keywords MMP en_US
dc.keywords Vascular Endothelial Cells en_US
dc.keywords Astrocytoma en_US
dc.description.bibliographiccitations Includes bibliographical references (leaves 53-62). en_US
dc.identifier.doi https://doi.org/10.26756/th.2015.38 en_US
dc.publisher.institution Lebanese American University en_US

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