Comparative genomic analysis of Staphylococcus aureus CC80 strains. (c2015)

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dc.contributor.author Bilen, Melhem E.
dc.date.accessioned 2016-01-29T10:59:12Z
dc.date.available 2016-01-29T10:59:12Z
dc.date.copyright 2015-05-07
dc.date.issued 2016-01-29
dc.identifier.uri http://hdl.handle.net/10725/2979
dc.description.abstract Staphylococcus aureus is a highly adaptive and versatile gram-positive bacterium and is considered one of the most isolated human pathogens and the most common cause of skin and soft tissue infections (SSTIs). The ongoing evolution of antimicrobial resistance namely the particularly methicillin-resistant strains of S. aureus (MRSA) has complicated the treatment of infections with such isolates. Global transmission of MRSA has been linked to international travel. However, in countries with a high prevalence of MRSA, imported cases are not easily distinguished from the domestic background prevalence. In this study we have selected ST80 MRSA strains isolated from clinical samples from Lebanon and Jordan for genome sequencing. DNA extracted from all isolates was prepared for sequencing, pooled together and run on Illumina MiSeq for paired-end 250-bp sequencing. Those isolates, found to be ST80, carried several virulence factors, namely PVL that causes a wide variety of diseases such as skin and mucosal lesions including necrotic hemorrhagic pneumonia. Additionally, intracellular adhesion proteins and clumping factors involved in biofilm formation and adhesion respectively were identified. Fibronectin binding proteins that play a role in invasion were expressed in all of our isolates. Moreover, exfoliative toxin, eta, which is the causative agent of staphylococcal scalded skin syndrome, was also detected. Nevertheless, different plasmids were identified in those isolates carrying several resistance genes towards Tetracyclines and Beta-lactams. Also, some isolates were shown to be resistant towards Lincosamide, Straptogramin and Macrolides. Finally, integrated phages, such as phi7401PVL and JS01 were identified in most of the isolates along with several CRISPR sequences that can be used in targeted therapies. Knowledge of epidemic MRSA clones can help in the development of effective strategies to aid in optimizing treatment, and revealing the mode of pathogenicity. The detection of rare SCCmec cassettes along with rare combination of toxin encoding genes, resistance factors and other virulence determinants could be indicative of MRSA clones being imported and warrants further studies to better understand and deal with such strains. en_US
dc.language.iso en en_US
dc.subject Staphylococcus aureus en_US
dc.subject Communicable diseases -- Pathogenesis en_US
dc.subject Virulence (Microbiology) -- Genetic aspects en_US
dc.subject Lebanese American University -- Dissertations en_US
dc.subject Dissertations, Academic en_US
dc.title Comparative genomic analysis of Staphylococcus aureus CC80 strains. (c2015) en_US
dc.type Thesis en_US
dc.term.submitted Spring en_US
dc.author.degree MS in Molecular Biology en_US
dc.author.school SAS en_US
dc.author.idnumber 201001355 en_US
dc.author.commembers Khazen, Georges
dc.author.commembers Khalaf, Roy
dc.author.commembers Nawas, Tarek
dc.author.woa OA en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.description.physdesc 1 hard copy: xiii, 63 leaves; col. ill.; 31 cm. available at RNL. en_US
dc.author.advisor Tokajian, Sima
dc.keywords Staphylococcus aureus en_US
dc.keywords MRSA en_US
dc.keywords ST80 en_US
dc.keywords Virulence determinants en_US
dc.keywords Resistance genes en_US
dc.description.bibliographiccitations Bibliography: leaves 36-50. en_US
dc.identifier.doi https://doi.org/10.26756/th.2015.34 en_US
dc.publisher.institution Lebanese American University en_US

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