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Kefir exhibits anti‑proliferative and pro‑apoptotic effects on colon adenocarcinoma cells with no significant effects on cell migration and invasion

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dc.contributor.author El-Sibai, Mirvat
dc.contributor.author Rizk, Sandra
dc.contributor.author Khoury, Nathalie
dc.contributor.author El-Hayek, Stephany
dc.contributor.author Tarras, Omayr
dc.contributor.author El Sabban, Marwan
dc.date.accessioned 2015-12-18T06:58:42Z
dc.date.available 2015-12-18T06:58:42Z
dc.date.copyright 2014
dc.date.issued 2015-12-18
dc.identifier.issn 1019-6439 en_US
dc.identifier.uri http://hdl.handle.net/10725/2844
dc.description.abstract Kefir, a fermented milk product, exhibits anti‑tumoral activity in vivo; yet its mechanism of action remains elusive. Recent studies have focused on the mechanism of action of kefir on cancer cells in vitro. The current study aims at examining the effect of kefir on cell survival, proliferation, and motility of colorectal cancer (CRC) cells. Kefir's anti‑cancer potential was tested on CRC cell lines, Caco‑2 and HT‑29, through cytotoxicity, proliferation, and apoptotic assays. The expression of certain genes involved in proliferation and apoptosis was measured using reverse transcriptase‑polymerase chain reaction (RT‑PCR) and western blotting. To assess the effect of kefir on cancer metastasis, wound‑healing and time‑lapse movies, in addition to collagen‑based invasion assay, were used. The results show that cell‑free fractions of kefir exhibit an anti‑proliferative effect on Caco‑2 and HT‑29 cells. Analysis of DNA content by flow cytometry revealed the ability of kefir to induce cell cycle arrest at the G1 phase. Kefir was also found to induce apoptosis, as seen by cell death ELISA. Results from RT‑PCR showed that kefir decreases the expression of transforming growth factor α (TGF‑α); and transforming growth factor‑β1 (TGF‑β1) in HT‑29 cells. Western blotting results revealed an upregulation in Bax:Bcl‑2 ratio, confirming the pro‑apoptotic effect of kefir, and an increase in p53 independent‑p21 expression upon kefir treatment. MMP expression was not altered by kefir treatment. Furthermore, results from time‑lapse motility movies, wound‑healing, and invasion assays showed no effect on the motility of colorectal as well as breast (MCF‑7 and MB‑MDA‑231) cancer cells upon kefir treatment. Our data suggest that kefir is able to inhibit the proliferation and induce apoptosis in HT‑29 and Caco‑2 CRC cells, yet it does not exhibit a significant effect on the motility and invasion of these cells in vitro. en_US
dc.language.iso en en_US
dc.title Kefir exhibits anti‑proliferative and pro‑apoptotic effects on colon adenocarcinoma cells with no significant effects on cell migration and invasion en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 200703859 en_US
dc.author.idnumber 199829370
dc.author.woa N/A en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal International Journal of Oncology en_US
dc.journal.volume 45 en_US
dc.journal.issue 5 en_US
dc.article.pages 2117-2127 en_US
dc.identifier.doi http://dx.doi.org/10.3892/ijo.2014.2635 en_US
dc.identifier.ctation Khoury, N., El‑Hayek, S., Tarras, O., El‑Sabban, M., El‑Sibai, M., & Rizk, S. (2014). Kefir exhibits anti‑proliferative and pro‑apoptotic effects on colon adenocarcinoma cells with no significant effects on cell migration and invasion. International journal of oncology, 45(5), 2117-2127. en_US
dc.author.email mirvat.elsibai@lau.edu.lb
dc.author.email sandra.rizk@lau.edu.lb
dc.identifier.url http://www.fasebj.org/content/28/1_Supplement/647.24.short


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