Daucus carota Pentane-Based Fractions Suppress Proliferation and Induce Apoptosis in Human Colon Adenocarcinoma HT-29 Cells by Inhibiting the MAPK and PI3K Pathways

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dc.contributor.author Shehaby, Wassim N.
dc.contributor.author Bodman-Smith, K.B.
dc.contributor.author Mansour, Anthony
dc.contributor.author Mroueh, Mohamad
dc.contributor.author Taleb, Robin I.
dc.contributor.author El-Sibai, Mirvat
dc.contributor.author Daher, Costantine F.
dc.date.accessioned 2015-12-17T13:58:16Z
dc.date.available 2015-12-17T13:58:16Z
dc.date.copyright 2015
dc.date.issued 2015-12-17
dc.identifier.issn 1096-620X en_US
dc.identifier.uri http://hdl.handle.net/10725/2828
dc.description.abstract Daucus carota L. ssp. carota (Apiacea, wild carrot, Queen Anne's lace) has been used in folk medicine throughout the world and recently was shown to possess anticancer and antioxidant activities. This study aims to determine the anticancer activity of the pentane fraction (F1) and the 1:1 pentane:diethyl ether fraction (F2) of the Daucus Carota oil extract (DCOE) against human colon adenocarcinoma cell lines (HT-29 and Caco-2). Treatment of cells with various concentrations of F1 or F2 fractions produced a dose-dependent inhibition of cell proliferation. Flow cytometric analysis indicated that both fractions induced sub-G1 phase accumulation and increased apoptotic cell death. Western blot revealed the activation of caspase-3, PARP cleavage, and a considerable increase in Bax and p53 levels, and a decrease in Bcl-2 level. Treatment of HT-29 cells with either fraction markedly decreased the levels of both phosphorylated Erk and Akt. Furthermore, the combined treatment of F1 or F2 with wortmannin showed no added inhibition of cell survival suggesting an effect of F1 or F2 through the phosphatidyl inositol 3-kinase (PI3K) pathway. This study proposes that DCOE fractions (F1 and F2) inhibit cell proliferation by inducing cell cycle arrest and apoptosis in HT-29 cells through the suppression of mitogen-activated protein kinase (MAPK)/Erk and PI3K/Akt pathways. en_US
dc.language.iso en en_US
dc.title Daucus carota Pentane-Based Fractions Suppress Proliferation and Induce Apoptosis in Human Colon Adenocarcinoma HT-29 Cells by Inhibiting the MAPK and PI3K Pathways en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SOP en_US
dc.author.school SAS
dc.author.idnumber 199590020 en_US
dc.author.idnumber 200901968
dc.author.idnumber 200703859
dc.author.idnumber 199190130
dc.author.woa N/A en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Journal of Medical Food en_US
dc.journal.volume 18 en_US
dc.journal.issue 7 en_US
dc.article.pages 745-752 en_US
dc.keywords Apoptosis en_US
dc.keywords Colon cancer en_US
dc.keywords Daucus carota en_US
dc.keywords MAPK en_US
dc.keywords PI3K en_US
dc.keywords Proliferation en_US
dc.identifier.doi https://doi.org/10.1089/jmf.2014.3225 en_US
dc.identifier.ctation Shebaby, W. N., Bodman-Smith, K. B., Mansour, A., Mroueh, M., Taleb, R. I., El-Sibai, M., & Daher, C. F. (2015). Daucus carota pentane-based fractions suppress proliferation and induce apoptosis in human colon adenocarcinoma HT-29 cells by inhibiting the MAPK and PI3K pathways. Journal of Medicinal Food, 18(7), 745-752. en_US
dc.author.email mmroueh@lau.edu.lb
dc.author.email robin.taleb@lau.edu.lb
dc.author.email mirvat.elsibai@lau.edu.lb
dc.author.email cdaher@lau.edu.lb
dc.identifier.url http://online.liebertpub.com/doi/abs/10.1089/jmf.2014.3225
dc.orcid.id https://orcid.org/0000-0001-8033-6951 en_US
dc.orcid.id https://orcid.org/0000-0003-4084-6759
dc.orcid.id https://orcid.org/0000-0002-8275-7263

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