IL-1 stimulates ceramide accumulation without inducing apoptosis in intestinal epithelial cells

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dc.contributor.author Homaidan, Fadia R.
dc.contributor.author El-Sabban, Marwan E.
dc.contributor.author Chakroun, Iman
dc.contributor.author El-Sibai, Mirvat
dc.contributor.author Dbaibo, Ghassan S.
dc.date.accessioned 2015-12-17T11:29:57Z
dc.date.available 2015-12-17T11:29:57Z
dc.date.copyright 2002
dc.date.issued 2016-04-11
dc.identifier.issn 0962-9351 en_US
dc.identifier.uri http://hdl.handle.net/10725/2820
dc.description.abstract Background: In inflammatory bowel disease (IBD), cytokine levels (such as interleukin-1 (IL-1)) are elevated. We have shown previously that IL-1 activates phospholipid signaling pathways in intestinal epithelial cells (IEC), leading to increased ceramide levels. Aim: To determine whether ceramide induces apoptosis in IEC. Methods: Apoptosis was evaluated by annexin-Vbinding or Hoechst nuclear staining. Levels of bcl-2, bcl-x, bax, p53 and p21 were determined by Western blotting, and cell cycle analysis was determined by flow cytometry. Results: IL-1 increased ceramide accumulation in a time-dependent and concentration-dependent manner with a peak response at 4 h, with [IL-1] = 30 ng/ml. Neither IL-1 nor ceramide induced apoptosis in IEC, but they increased bcl-2 levels and decreased bax and p21 levels without affecting bcl-x and p53 levels. They also caused a slight but significant increase in the G2/M phase. These data suggest a role for ceramide in IBD and suggest a possible mechanism for the enhanced tumorigenic activity in IBD patients. en_US
dc.language.iso en en_US
dc.title IL-1 stimulates ceramide accumulation without inducing apoptosis in intestinal epithelial cells en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 200703859 en_US
dc.author.woa N/A en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Mediators of Inflammation en_US
dc.journal.volume 11 en_US
dc.journal.issue 1 en_US
dc.article.pages 39-45 en_US
dc.keywords Epithelial cells en_US
dc.keywords IL-1 en_US
dc.keywords Bcl-2 en_US
dc.keywords Bcl-x en_US
dc.keywords Bax en_US
dc.keywords IBD en_US
dc.keywords Inflammation en_US
dc.identifier.doi http://dx.doi.org/10.1080/09629350210313 en_US
dc.identifier.ctation Homaidan, F. R., El-Sabban, M. E., Chakroun, I., El-Sibai, M., & Dbaibo, G. S. (2002). IL-1 stimulates ceramide accumulation without inducing apoptosis in intestinal epithelial cells. Mediators of inflammation, 11(1), 39-45. en_US
dc.author.email mirvat.elsibai@lau.edu.lb
dc.identifier.url http://www.hindawi.com/journals/mi/2002/467015/abs/
dc.orcid.id https://orcid.org/0000-0003-4084-6759 en_US

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