Daucus carota pentane-based fractions arrest the cell cycle and increase apoptosis in MDA-MB-231 breast cancer cells

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dc.contributor.author Shebaby, Wassim N.
dc.contributor.author Mroueh, Mohammad
dc.contributor.author Bodman-Smith, Kikki
dc.contributor.author Mansour, Anthony
dc.contributor.author Taleb, Robin I.
dc.contributor.author Daher, Costantine F.
dc.contributor.author El-Sibai, Mirvat
dc.date.accessioned 2015-12-16T09:47:44Z
dc.date.available 2015-12-16T09:47:44Z
dc.date.copyright 2014
dc.date.issued 2015-12-16
dc.identifier.issn 1472-6882 en_US
dc.identifier.uri http://hdl.handle.net/10725/2819
dc.description.abstract Background Daucus carota L.ssp.carota (wild carrot), an herb used in folk medicine worldwide, was recently demonstrated to exhibit anticancer activity. In this study we examined the anticancer effect of Daucus carota oil extract (DCOE) fractions on the human breast adenocarcinoma cell lines MDA-MB-231 and MCF-7 and clarified the mechanism of action. Methods and results Using the WST assay, the pentane fraction (F1) and 1:1 pentane:diethyl ether fraction (F2) were shown to possess the highest cytotoxicity against both cell lines. Flow cytometric analysis revealed that both fractions induced the accumulation of cells in the sub-G1 phase, increase in apoptotic cell death and chromatin condensation. The increase in apoptosis in response to treatment was also apparent in the increase in BAX and the decrease in Bcl-2 levels as well as the proteolytic cleavage of both caspase-3 and PARP as revealed by Western blot. Furthermore, treatment of MDA-MB-231 cells with either fraction significantly reduced the level of phosphorylated Erk but did not show any effect on phosphorylated Akt. The combined treatment with a potent PI3K inhibitor (wortmannin) and F1 or F2 fraction had a synergistic inhibitory effect on cell survival which shows that these two drugs work on different pathways. Conclusions These results suggest that the pentane-based fractions of DCOE possess potential anti-cancer activity that is mainly mediated through the Erk pathway. en_US
dc.language.iso en en_US
dc.title Daucus carota pentane-based fractions arrest the cell cycle and increase apoptosis in MDA-MB-231 breast cancer cells en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.school SOP
dc.author.idnumber 199590020
dc.author.idnumber 200901968
dc.author.idnumber 199190130
dc.author.idnumber 200703859
dc.author.woa N/A en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal BMC Complementary and Alternative Medicine en_US
dc.journal.volume 14 en_US
dc.journal.issue 387 en_US
dc.keywords Daucus carota en_US
dc.keywords Breast cancer en_US
dc.keywords Proliferation en_US
dc.keywords Apoptosis en_US
dc.keywords PI3K en_US
dc.keywords MAPK en_US
dc.identifier.doi http://dx.doi.org/10.1186/1472-6882-14-387 en_US
dc.identifier.ctation Shebaby, W. N., Mroueh, M., Bodman-Smith, K., Mansour, A., Taleb, R. I., Daher, C. F., & El-Sibai, M. (2014). Daucus carota pentane-based fractions arrest the cell cycle and increase apoptosis in MDA-MB-231 breast cancer cells. BMC complementary and alternative medicine, 14(1), 387. en_US
dc.author.email mmroueh@lau.edu.lb
dc.author.email robin.taleb@lau.edu.lb
dc.author.email cdaher@lau.edu.lb
dc.author.email mirvat.elsibai@lau.edu.lb
dc.identifier.url http://bmccomplementalternmed.biomedcentral.com/articles/10.1186/1472-6882-14-387
dc.orcid.id https://orcid.org/0000-0003-4084-6759 en_US

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