Antioxidant and hepatoprotective activities of the oil fractions from wild carrot (Daucus carota ssp. carota)

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dc.contributor.author Shebaby, Wassim N.
dc.contributor.author Daher, Costantine F.
dc.contributor.author El-Sibai, Mirvat
dc.contributor.author Bodman-Smith, Kikki
dc.contributor.author Mansour, Anthony
dc.contributor.author Karam, Marc C.
dc.contributor.author Mroueh, Mohamad
dc.date.accessioned 2015-12-16T07:52:44Z
dc.date.available 2015-12-16T07:52:44Z
dc.date.copyright 2015
dc.date.issued 2015-12-16
dc.identifier.issn 1388-0209 en_US
dc.identifier.uri http://hdl.handle.net/10725/2815
dc.description.abstract Context: Wild carrot, Daucus carota L. ssp. carota (Apiacae), is widely distributed throughout the world and has various uses in traditional medicine in Lebanon. Objective: The present study aimed to fractionate and analyze the chemical composition of the Daucus carota oil extract (DCOE) fractions and to evaluate their antioxidant and hepatoprotective properties in vitro and in vivo. Materials and methods: DCOE was chromatographed on silica gel column to produce four fractions: pentane (F1), 50:50 pentane:diethyl ether (F2), diethyl ether (F3), and 93:7 chloroform: methanol (F4). Qualitative and quantitative analyses of oil fractions were performed by GC-MS and HPLC techniques. The in vitro antioxidant properties were assessed using DPPH, FIC, and ferric-reducing antioxidant power (FRAP) assays. The hepatoprotective property was determined by examining the levels of serum markers (alanine transaminase (ALT) and aspartate transaminase (AST)) and hepatic antioxidant (superoxide dismutase (SOD), catalase (CAT), and glutathione-S-transferase (GST)) enzymes in CCl4-intoxicated mice pretreated with intraperitoenal 50, 100, or 200 mg/kg b.w. of the oil fractions for 5 d. Results: GCMS analysis of F2 revealed the presence of 2-himachalen-6-ol (61.4%) which is reported for the first time in Daucus carota species. F3 and F4 were rich in phenolics and flavonoids and demonstrated significant DPPH activity (IC50 = 0.29 and 0.38 mg/ml, respectively) and high FRAP values (225.11 and 437.59 µmol FeSO4/g, respectively). The sesquiterpene-rich fraction F1 had the highest FIC ability (IC50 = 0.28 mg/ml). Pretreatment with F1 and F4 reversed the CCl4-induced decrease in SOD, CAT, and GST levels and reduced significantly hepatic damage. Discussion and conclusion: The current results suggested that wild carrot oil fractions exhibited a unique chemical composition and possessed significant antioxidant activities as well as hepatoprotective effects against CCl4-induced hepatotoxicity. en_US
dc.language.iso en en_US
dc.title Antioxidant and hepatoprotective activities of the oil fractions from wild carrot (Daucus carota ssp. carota) en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.school SOP
dc.author.idnumber 199190130 en_US
dc.author.idnumber 200703859
dc.author.idnumber 199590020
dc.author.woa N/A en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Pharmaceutical Biology en_US
dc.journal.volume 53 en_US
dc.journal.issue 9 en_US
dc.article.pages 1285-1294 en_US
dc.keywords CAT en_US
dc.keywords DPPH en_US
dc.keywords FRAP en_US
dc.keywords FIC en_US
dc.keywords Flavonoids en_US
dc.keywords GST en_US
dc.keywords Phenolics en_US
dc.keywords SOD en_US
dc.identifier.doi http://dx.doi.org/10.3109/13880209.2014.976349 en_US
dc.identifier.ctation Shebaby, W. N., Daher, C. F., El-Sibai, M., Bodman-Smith, K., Mansour, A., Karam, M. C., & Mroueh, M. (2015). Antioxidant and hepatoprotective activities of the oil fractions from wild carrot (Daucus carota ssp. carota). Pharmaceutical biology, 53(9), 1285-1294. en_US
dc.author.email cdaher@lau.edu.lb
dc.author.email mirvat.elsibai@lau.edu.lb
dc.author.email mmroueh@lau.edu.lb
dc.identifier.url http://www.tandfonline.com/doi/abs/10.3109/13880209.2014.976349
dc.orcid.id https://orcid.org/0000-0003-4084-6759 en_US

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