The modulatory effect of leptin on the overall insulin production in ex-vivo normal rat pancreas

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dc.contributor.author Daher, C.
dc.contributor.author Haddad, N.
dc.contributor.author Howland, R.
dc.contributor.author Baroody, G.
dc.date.accessioned 2015-12-16T07:24:33Z
dc.date.available 2015-12-16T07:24:33Z
dc.date.copyright 2006
dc.date.issued 2015-12-16
dc.identifier.issn 0008-4212 en_US
dc.identifier.uri http://hdl.handle.net/10725/2812
dc.description.abstract Leptin has a modulator effect on glucose-stimulated insulin secretion. To define the influences of different glucose (4, 8, 12, and 16 mmol/L) and leptin (5, 10, 15, and 20 nmol/L) concentrations on total insulin release in ex vivo pancreatic preparations, a customized perfusion technique was used. Such a profile of concentration brought about an index for the combined effect of leptin and glucose on the production of insulin. Insulin output was measured by radioimmunoassay. Stimulated by glucose alone in the control group, insulin secretion confirmed a bi-phasic pattern. Addition of leptin in the experimental group suppressed insulin secretion compared with control. A U-shape pattern of suppression was observed when the leptin and stimulatory glucose concentrations were combined. At 12 mmol/L glucose, leptin showed maximal insulin suppression. Leptin’s effect on insulin was glucose dependent and showed a reproducible U-shaped pattern of suppression, which implicated possible direct dose-dependent interaction between leptin and glucose on insulin secretion. en_US
dc.language.iso en en_US
dc.title The modulatory effect of leptin on the overall insulin production in ex-vivo normal rat pancreas en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 199190130 en_US
dc.author.woa N/A en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Canadian Journal of Physiology and Pharmacology en_US
dc.journal.volume 84 en_US
dc.journal.issue 2 en_US
dc.article.pages 157-162 en_US
dc.keywords Leptin en_US
dc.keywords Insulin en_US
dc.keywords Glucose en_US
dc.keywords Ex-vivo en_US
dc.keywords Radioimmunoassay en_US
dc.identifier.doi http://dx.doi.org/10.1139/y06-006 en_US
dc.identifier.ctation Haddad, N., Howland, R., Baroody, G., & Daher, C. (2006). The modulatory effect of leptin on the overall insulin production in ex-vivo normal rat pancreas. Canadian journal of physiology and pharmacology, 84(2), 157-162. en_US
dc.author.email cdaher@lau.edu.lb
dc.identifier.url http://www.nrcresearchpress.com/doi/abs/10.1139/y06-006#.V44HR7h97y0

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