Postprandial hyperaminoacidaemia overcomes insulin resistance of protein anabolism in men with type 2 diabetes

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dc.contributor.author Bassil, M.
dc.contributor.author Morais, J.A.
dc.contributor.author Pereira, S.
dc.contributor.author Chevalier, S.
dc.contributor.author Gougeon, R.
dc.contributor.author Marliss, J.
dc.date.accessioned 2015-12-04T09:18:07Z
dc.date.available 2015-12-04T09:18:07Z
dc.date.copyright 2011
dc.date.issued 2015-12-04
dc.identifier.issn 0012-186X en_US
dc.identifier.uri http://hdl.handle.net/10725/2757
dc.description.abstract Aims/hypothesis Although protein is usually ignored when considering insulin resistance, we have shown resistance of protein concurrent with glucose metabolism in men with type 2 diabetes during a hyperinsulinaemic clamp at euglycaemia and fasting aminoacidaemia. We hypothesised that this resistance is even worse during conditions that simulate the postprandial state, when anabolism should be maximal. Methods Eight overweight and obese men with type 2 diabetes underwent a hyperinsulinaemic–hyperglycaemic (8 mmol/l) clamp, first with plasma amino acids at postabsorptive (Hyper-2) then at postprandial concentrations (Hyper-3). Whole-body protein kinetics were assessed using l-[1-13C]leucine. Hyper-2 results were compared with those of diabetic men whose plasma glucose was lowered to 5.5 mmol/l and fasting aminoacidaemia maintained during the hyperinsulinaemic clamp (Hyper-1). Results In Hyper-2 vs Hyper-1 clamps, leucine flux (2.99 ± 0.16 vs 2.62 ± 0.06 μmol kg [fat-free mass (FFM)]−1 min−1), rates of synthesis (2.31 ± 0.15 vs 1.98 ± 0.06) and breakdown (2.38 ± 0.16 vs 2.00 ± 0.07) were higher (p < 0.05), but leucine oxidation and net balance did not differ. In Hyper-3 vs Hyper-2 clamps, leucine flux and synthesis and oxidation rates increased markedly as did net balance (0.84 ± 0.09 vs −0.07 ± 0.04 μmol [kg FFM]−1 min−1, p < 0.0001). Conclusions/interpretation In type 2 diabetic men, insulin resistance of protein metabolism is of the same magnitude at 8 vs 5.5 mmol/l, but turnover rates are higher with hyperglycaemia. Contrary to our hypothesis, sustained postprandial-level hyperaminoacidaemia stimulated positive net protein balance comparable with that previously found in lean non-diabetic men. This was sufficient to overcome the insulin resistance of protein anabolism. en_US
dc.language.iso en en_US
dc.title Postprandial hyperaminoacidaemia overcomes insulin resistance of protein anabolism in men with type 2 diabetes en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 201102356 en_US
dc.author.woa N/A en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Diabetologia en_US
dc.journal.volume 54 en_US
dc.journal.issue 3 en_US
dc.article.pages 648-656 en_US
dc.keywords Fed state en_US
dc.keywords Hyperaminoacidaemia en_US
dc.keywords Hyperinsulinaemic clamp en_US
dc.keywords Insulin resistance en_US
dc.keywords Leucine en_US
dc.keywords Kinetics en_US
dc.keywords Protein metabolism en_US
dc.keywords Type 2 diabetes en_US
dc.identifier.doi http://dx.doi.org/10.1007/s00125-010-1980-9 en_US
dc.identifier.ctation Bassil, M., Marliss, E. B., Morais, J. A., Pereira, S., Chevalier, S., & Gougeon, R. (2011). Postprandial hyperaminoacidaemia overcomes insulin resistance of protein anabolism in men with type 2 diabetes. Diabetologia, 54(3), 648-656. en_US
dc.author.email mbassil@lau.edu.lb
dc.identifier.url http://link.springer.com/article/10.1007/s00125-010-1980-9

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