dc.contributor.author |
Bekdash, Amira |
|
dc.contributor.author |
Abi-Habib, Ralph J. |
|
dc.contributor.author |
Tanios, Rita |
|
dc.contributor.author |
Stone, Everett |
|
dc.contributor.author |
Georgiou, Georges |
|
dc.contributor.author |
Frankel, Arthur E. |
|
dc.contributor.author |
Kassab, Elias |
|
dc.date.accessioned |
2015-12-01T08:20:38Z |
|
dc.date.available |
2015-12-01T08:20:38Z |
|
dc.date.copyright |
2013 |
|
dc.date.issued |
2015-12-01 |
|
dc.identifier.issn |
0145-2126 |
en_US |
dc.identifier.uri |
http://hdl.handle.net/10725/2741 |
|
dc.description.abstract |
In this study, we target arginine auxotrophy of AML cell lines using human arginase I cobalt-PEG5000. HuArgI(Co)-PEG5000 was cytotoxic to all AML cell lines tested. Mononuclear cells and CD34+ blasts were not sensitive demonstrating the selectivity of HuArgI(Co)-PEG5000-induced arginine deprivation. Addition of l-citrulline led to the rescue of five cell lines. The four cell lines that were not rescued by l-citrulline did not express argininosuccinate synthetase-1, indicating complete arginine auxotrophy. Inhibition of autophagy increased cell sensitivity to HuArgI(Co)-PEG5000 demonstrating the protective role of autophagy following arginine deprivation.
We have shown that AML can be selectively targeted using HuArgI(Co)-PEG5000-induced arginine depletion. |
en_US |
dc.language.iso |
en |
en_US |
dc.title |
Human recombinant arginase I(Co)-PEG5000 [HuArgI(Co)-PEG5000]-induced arginine depletion is selectively cytotoxic to human acute myeloid leukemia cells |
en_US |
dc.type |
Article |
en_US |
dc.description.version |
Published |
en_US |
dc.author.school |
SAS |
en_US |
dc.author.idnumber |
201004441 |
en_US |
dc.author.idnumber |
200901419 |
|
dc.author.woa |
N/A |
en_US |
dc.author.department |
Natural Sciences |
en_US |
dc.description.embargo |
N/A |
en_US |
dc.relation.journal |
Leukemia Research |
en_US |
dc.journal.volume |
37 |
en_US |
dc.journal.issue |
11 |
en_US |
dc.article.pages |
1565-1571 |
en_US |
dc.keywords |
HuArgI(Co)-PEG5000 |
en_US |
dc.keywords |
AML |
en_US |
dc.keywords |
Arginine |
en_US |
dc.keywords |
Autophagy |
en_US |
dc.identifier.doi |
http://dx.doi.org/10.1016/j.leukres.2013.08.007 |
en_US |
dc.identifier.ctation |
Tanios, R., Bekdash, A., Kassab, E., Stone, E., Georgiou, G., Frankel, A. E., & Abi-Habib, R. J. (2013). Human recombinant arginase I (Co)-PEG5000 [HuArgI (Co)-PEG5000]-induced arginine depletion is selectively cytotoxic to human acute myeloid leukemia cells. Leukemia research, 37(11), 1565-1571. |
en_US |
dc.author.email |
amira.bekdash@lau.edu.lb |
|
dc.author.email |
ralph.abihabib@lau.edu.lb |
|
dc.identifier.url |
http://www.sciencedirect.com/science/article/pii/S0145212613002889 |
|