The Development and Characterization of a Human Midgut Carcinoid Cell Line

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dc.contributor.author Van Burenn II, George
dc.contributor.author Rashid, Asif
dc.contributor.author Yang, Anthony
dc.contributor.author Abdalla, Eddie
dc.contributor.author Gray, Micheal
dc.contributor.author Liu, Wenbiao
dc.contributor.author Somicio, Ray
dc.contributor.author Fan, Fan
dc.contributor.author Camp, Ramsay
dc.contributor.author Yao, James
dc.contributor.author Ellis, Lee
dc.date.accessioned 2015-11-16T11:03:40Z
dc.date.available 2015-11-16T11:03:40Z
dc.date.copyright 2007
dc.date.issued 2015-11-16
dc.identifier.issn 1078-0432 en_US
dc.identifier.uri http://hdl.handle.net/10725/2579
dc.description.abstract Purpose: Gastrointestinal neuroendocrine tumors (NET) are rare heterogeneous tumors that hypersecrete neuropeptides. The scarcity of good gastrointestinal NET models has limited the ability to study potential therapeutic agents. We describe and characterize the establishment of a human midgut carcinoid tumor cell line carcinoid tumor 2 (CNDT2). Experimental Design: Tumor cells (CNDT2) were isolated from a liver metastasis from a patient with a primary ileal carcinoid. After 9 weeks in culture, the cells were plated in soft agar, and cells from a single colony were put back in culture (CNDT2.1). Those CNDT2.1 cells were injected s.c. into nude mice. Cells were isolated from a single resultant tumor (CNDT2.5), cultured, and characterized by electron microscopy, reverse transcription-PCR, serotonin enzyme immunoassay, Western blotting, and immunohistochemical analysis for NET markers and potential therapeutic targets. Results: CNDT2 cells grew in monolayers in vitro, formed colonies in soft agar, and formed tumors in mice. Electron microscopy revealed round, pleomorphic, electron-dense neurosecretory granules characteristic of NETs. Tumor xenografts exhibited the appearance of NETs with small “salt-and-pepper” nuclei on H&E staining and chromogranin A, synaptophysin, and CD56 on immunohistochemical staining. CNDT2.5 cells produced serotonin and expressed insulin-like growth factor receptor-I, platelet-derived growth factor receptor-β, vascular endothelial growth factor receptor-1, cMET, epidermal growth factor receptor, neuropilin-1, and somatostatin receptors 1 to 5. Cytogenetic analysis revealed the presence of deletions at 2p and 6q and numerous translocations. Conclusion: The establishment of this human midgut carcinoid tumor cell line may serve as a useful model system for studying cell biology and novel targeted agents in preclinical models. en_US
dc.language.iso en en_US
dc.title The Development and Characterization of a Human Midgut Carcinoid Cell Line en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SOM en_US
dc.author.idnumber 20110945 en_US
dc.author.woa N/A en_US
dc.author.department N/A en_US
dc.description.embargo N/A en_US
dc.relation.journal Clinical cancer research en_US
dc.journal.volume 13 en_US
dc.article.pages 4704-4712 en_US
dc.keywords Carcinoid en_US
dc.keywords Neuroendocrine en_US
dc.keywords Secretory granule en_US
dc.keywords Growth factor en_US
dc.keywords Serotonin en_US
dc.identifier.doi http://dx.doi.org/ 10.1158/1078-0432.CCR-06-2723 en_US
dc.identifier.ctation Van Buren, G., Rashid, A., Yang, A. D., Abdalla, E. K., Gray, M. J., Liu, W., ... & Ellis, L. M. (2007). The development and characterization of a human midgut carcinoid cell line. Clinical Cancer Research, 13(16), 4704-4712. en_US
dc.author.email eddie.abdalla@lau.edu.lb

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