Cofilin determines the migration behavior and turning frequency of metastatic cancer cells

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dc.contributor.author Sidani, Mazen
dc.contributor.author Wessels, Deborah
dc.contributor.author Mouneimne, Ghassan
dc.contributor.author Ghosh, Mousumi
dc.contributor.author Goswami, Sumanta
dc.contributor.author Sarmiento, Corina
dc.contributor.author Wang, Weigang
dc.contributor.author Kuhl, Spencer
dc.contributor.author El-Sibai, Mirvat
dc.contributor.author Backer, Jonathan M.
dc.contributor.author Eddy, Robert
dc.contributor.author Soll, David
dc.contributor.author Condeelis, John
dc.date.accessioned 2015-11-09T13:51:56Z
dc.date.available 2015-11-09T13:51:56Z
dc.date.copyright 2007-11-19
dc.date.issued 2015-11-09
dc.identifier.issn 0021-9525 en_US
dc.identifier.uri http://hdl.handle.net/10725/2501
dc.description.abstract We have investigated the effects of inhibiting the expression of cofilin to understand its role in protrusion dynamics in metastatic tumor cells, in particular. We show that the suppression of cofilin expression in MTLn3 cells (an apolar randomly moving amoeboid metastatic tumor cell) caused them to extend protrusions from only one pole, elongate, and move rectilinearly. This remarkable transformation was correlated with slower extension of fewer, more stable lamellipodia leading to a reduced turning frequency. Hence, the loss of cofilin caused an amoeboid tumor cell to assume a mesenchymal-type mode of movement. These phenotypes were correlated with the loss of uniform chemotactic sensitivity of the cell surface to EGF stimulation, demonstrating that to chemotax efficiently, a cell must be able to respond to chemotactic stimulation at any region on its surface. The changes in cell shape, directional migration, and turning frequency were related to the re-localization of Arp2/3 complex to one pole of the cell upon suppression of cofilin expression. en_US
dc.language.iso en en_US
dc.title Cofilin determines the migration behavior and turning frequency of metastatic cancer cells en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 200703859 en_US
dc.author.woa N/A en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal The Journal of Cell Biology en_US
dc.journal.volume 179 en_US
dc.journal.issue 4 en_US
dc.article.pages 777-791 en_US
dc.identifier.doi http://dx.doi.org/10.1083/jcb.200707009 en_US
dc.identifier.ctation Sidani, M., Wessels, D., Mouneimne, G., Ghosh, M., Goswami, S., Sarmiento, C., ... & Condeelis, J. (2007). Cofilin determines the migration behavior and turning frequency of metastatic cancer cells. The Journal of cell biology, 179(4), 777-791. en_US
dc.author.email mirvat.elsibai@lau.edu.lb
dc.identifier.url http://jcb.rupress.org/content/179/4/777.short
dc.orcid.id https://orcid.org/0000-0003-4084-6759 en_US

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