Phospholipase C negatively regulates Rac/Cdc42 activation in antigen-stimulated mast cells

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dc.contributor.author El-Sibai, Mirvat
dc.contributor.author Backer, Jonathan M.
dc.date.accessioned 2015-11-09T13:21:49Z
dc.date.available 2015-11-09T13:21:49Z
dc.date.copyright 2007-01
dc.date.issued 2015-11-09
dc.identifier.issn 0014-2980 en_US
dc.identifier.uri http://hdl.handle.net/10725/2497
dc.description.abstract The Rho GTPases Rac and Cdc42 play a central role in the regulation of secretory and cytoskeletal responses in antigen-stimulated mast cells. In this study, we examine the kinetics and mechanism of Rac and Cdc42 activation in the rat basophilic leukemia RBL- 2H3 cells. The activation kinetics of both Rac and Cdc42 show a biphasic profile, consisting of an early transient peak at 1 min and a late sustained activation phase at 20–40 min. The inhibition of phospholipase C (PLC)c causes a twofold increase in Rac and Cdc42 activation that coincides with a dramatic production of atypical filopodialike structures. Inhibition of protein kinase C using bisindolylmaleimide mimics the effect of PLCc inhibition on Rac activation, but not on Cdc42 activation. In contrast, depletion of intracellular calcium leads to a complete inhibition of the early activation peak of both Rac and Cdc42, without significant effects on the late sustained activation. These data suggest that PLCc is involved in a negative feedback loop that leads to the inhibition of Rac and Cdc42. They also suggest that the presence of intracellular calcium is a prerequisite for both Rac and Cdc42 activation. en_US
dc.language.iso en en_US
dc.title Phospholipase C negatively regulates Rac/Cdc42 activation in antigen-stimulated mast cells en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 200703859 en_US
dc.author.woa N/A en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal European Journal of Immunology en_US
dc.journal.volume 37 en_US
dc.journal.issue 1 en_US
dc.article.pages 261-270 en_US
dc.keywords Calcium en_US
dc.keywords Cdc42 en_US
dc.keywords Mast cells en_US
dc.keywords Phospholipase en_US
dc.keywords C en_US
dc.keywords Rac en_US
dc.identifier.doi http://dx.doi.org/10.1002/eji.200635875 en_US
dc.identifier.ctation El‐Sibai, M., & Backer, J. M. (2007). Phospholipase Cγ negatively regulates Rac/Cdc42 activation in antigen‐stimulated mast cells. European journal of immunology, 37(1), 261-270. en_US
dc.author.email mirvat.elsibai@lau.edu.lb
dc.identifier.url http://onlinelibrary.wiley.com/doi/10.1002/eji.200635875/full
dc.orcid.id https://orcid.org/0000-0003-4084-6759 en_US

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