The antioxidant and anticancer effects of Wild Carrot oil extract

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dc.contributor.author Shebaby, Wassim Nasri
dc.contributor.author El-Sibai, Mirvat
dc.contributor.author Bodman-Smith, Kikki
dc.contributor.author Karam, Marc Christoph
dc.contributor.author Mroueh, Mohamad
dc.contributor.author Daher, Costantine F.
dc.date.accessioned 2015-11-04T11:14:39Z
dc.date.available 2015-11-04T11:14:39Z
dc.date.copyright 2012-07-20
dc.date.issued 2015-11-04
dc.identifier.issn 0951-418X en_US
dc.identifier.uri http://hdl.handle.net/10725/2433
dc.description.abstract Daucus carota L. ssp. carota (Apiacea) is used in traditional medicine in Lebanon and in different regions throughout the world. The present study investigates the in vitro anticancer activities of Daucus carota oil extract (DCOE) on four human cancer cell lines as well as its in vitro antioxidant activity. DCOE was extracted from the dried umbels with 50:50 acetone-methanol. The oil extract was analyzed by gas chromatography–mass spectrometry and screened for its antioxidant properties in vitro using 1,1-diphenyl-2-picryl hydrazyl free radical scavenging assay (DPPH), ferrous ion chelating assay (FIC) and the ferric reducing antioxidant power assay (FRAP). The anticancer activity of the oil extract against human colon (HT-29, Caco-2) and breast (MCF-7, MDA-MB-231) cancer cell lines was evaluated using the trypan blue exclusion method and the WST-1 cell proliferation assay. DCOE exhibited antioxidant activity in all assays used. The FRAP value was 164 ± 5.5 µmol FeSO4/g, and the IC50 values for DPPH and FIC assays were 2.1 ± 0.03 mg/ml and 0.43 ± 0.02 mg/ml, respectively. Also, DCOE demonstrated a significant increase in cell death and decrease in cell proliferation. The effect of DCOE on the cell lines exhibited time and dose-dependent responses. The present study established that DCOE possesses both antioxidant and promising anticancer activities. en_US
dc.language.iso en en_US
dc.title The antioxidant and anticancer effects of Wild Carrot oil extract en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.school SOP
dc.author.idnumber 200703859 en_US
dc.author.idnumber 199590020
dc.author.idnumber 199190130
dc.author.woa N/A en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Phytotherapy Research en_US
dc.journal.volume 27 en_US
dc.journal.issue 5 en_US
dc.article.pages 737-744 en_US
dc.keywords Daucus carota en_US
dc.keywords Colon cancer en_US
dc.keywords Breast cancer en_US
dc.keywords Antioxidant en_US
dc.keywords Anticancer en_US
dc.identifier.doi http://dx.doi.org/10.1002/ptr.4776 en_US
dc.identifier.ctation Shebaby, W. N., El‐Sibai, M., Smith, K. B., Karam, M. C., Mroueh, M., & Daher, C. F. (2013). The antioxidant and anticancer effects of wild carrot oil extract. Phytotherapy Research, 27(5), 737-744. en_US
dc.author.email mirvat.elsibai@lau.edu.lb
dc.author.email mmroueh@lau.edu.lb
dc.author.email cdaher@lau.edu.lb
dc.identifier.url http://onlinelibrary.wiley.com/doi/10.1002/ptr.4776/full
dc.orcid.id https://orcid.org/0000-0003-4084-6759 en_US

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