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Proliferation and differentiation of human adipose-derived mesenchymal stem cells (ASCs) into osteoblastic lineage are passage dependent

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dc.contributor.author Faour, Wissam
dc.contributor.author Abdalla, Eddie
dc.contributor.author Di Battista, Jiovanni
dc.contributor.author Shehaby, Wassim
dc.contributor.author Kizilay, Ozge
dc.contributor.author Hamade, Eva
dc.contributor.author Abou Merhi, Raghida
dc.contributor.author Mebarek, Saida
dc.contributor.author Abdallah, Dina
dc.contributor.author Badran, Bassam
dc.contributor.author Saad, Fady
dc.date.accessioned 2015-10-27T09:32:40Z
dc.date.available 2015-10-27T09:32:40Z
dc.date.copyright 2014
dc.date.issued 2015-10-27
dc.identifier.issn 1023-3830 en_US
dc.identifier.uri http://hdl.handle.net/10725/2354
dc.description.abstract Objective The effect of in vitro expansion of human adipose-derived stem cells (ASCs) on stem cell properties is controversial. We examined serial subcultivation with expansion on the ability of ASCs to grow and differentiate into osteoblastic lineages. Design Flow cytometric analysis, growth kinetics, cell population doubling time, light microscopy and confocal analysis, and osteogenesis induction were performed to assess growth and osteogenic potential of subcultivated ASCs at passages 2 (P2), P4 and P6. Results Flow cytometric analysis revealed that ASCs at P2 express classical mesenchymal stem cell markers including CD44, CD73, and CD105, but not CD14, CD19, CD34, CD45, or HLA-DR. Calcium deposition and alkaline phosphatase activity were the highest at P2 but completely abrogated at P4. Increased passage number impaired cell growth; P2 cultures exhibited exponential growth, while cells at P4 and P6 showed near linear growth with cell population doubling times increased from 3.2 at P2 to 4.8 d at P6. Morphologically, cells in various subcultivation stages showed flattened shape at low density but spindle-like structures at confluency as judged by phalloidin staining. Conclusions Osteogenic potential of ASCs is impaired by successive passaging and may not serve as a useful clinical source of osteogenic ASCs past P2. en_US
dc.language.iso en en_US
dc.title Proliferation and differentiation of human adipose-derived mesenchymal stem cells (ASCs) into osteoblastic lineage are passage dependent en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SOM en_US
dc.author.idnumber 200904962 en_US
dc.author.idnumber 201100945
dc.author.woa N/A en_US
dc.author.department N/A en_US
dc.description.embargo N/A en_US
dc.relation.journal Inflammation Research en_US
dc.journal.volume 63 en_US
dc.journal.issue 11 en_US
dc.article.pages 907-917 en_US
dc.keywords Stem cells en_US
dc.keywords Osteogenesis en_US
dc.keywords Growth kinetics en_US
dc.keywords Adipose tissues en_US
dc.keywords Differentiation en_US
dc.identifier.doi http://dx.doi.org/10.1007/s00011-014-0764-y en_US
dc.identifier.ctation Di Battista, J. A., Shebaby, W., Kizilay, O., Hamade, E., Merhi, R. A., Mebarek, S., ... & Faour, W. H. (2014). Proliferation and differentiation of human adipose-derived mesenchymal stem cells (ASCs) into osteoblastic lineage are passage dependent. Inflammation Research, 63(11), 907-917. en_US
dc.author.email wissam.faour@lau.edu.lb
dc.author.email eddie.abdalla@lau.edu.lb


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