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A novel xylene-free deparaffinization method for the extraction of proteins from human derived formalin-fixed paraffin embedded (FFPE) archival tissue blocks

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dc.contributor.author Mansour, Anthony
dc.contributor.author Chatila, Rajaa
dc.contributor.author Bejjani, Noha
dc.contributor.author Faour, Wissam H.
dc.contributor.author Dagher, Carole
dc.date.accessioned 2015-10-27T09:19:12Z
dc.date.available 2015-10-27T09:19:12Z
dc.date.copyright 2014
dc.date.issued 2015-10-27
dc.identifier.issn 2215-0161 en_US
dc.identifier.uri http://hdl.handle.net/10725/2353
dc.description.abstract Protein detection methods in formalin-fixed paraffin embedded (FFPE) tissue blocks are widely used in research and clinical setting in order to diagnose or to confirm a diagnosis of various types of diseases. Therefore, multiple protein extraction methods from FFPE tissue sections have been developed in this regard. However, the yield and the quality of proteins extracted from FFPE tissues are significantly reduced in blocks stored for longer periods of time. Regardless the protein extraction method used, tissue sections must be first deparaffinized with xylene, and then washed in serial dilutions of ethanol in order to remove the toxic organic solvent “xylene” and rehydrate the tissue. The objective of this study was first to develop a method to deparaffinize FFPE blocks that excludes the use of toxic solvent “xylene”. Second minimize the time required to perform the extraction. Here we describe a method where: • The entire paraffin embedded blocks are deparaffinized and rehydrated using only hot distilled water as a substitute for both xylene and ethanol • The entire procedure takes about 15 min • Deparaffinized blocks are immediately homogenized in lysis buffer, and the obtained lysate analyzed by Western blot. With this new modified technique, we were able to successfully detect actin and AKT proteins in lysates from blocks embedded in paraffin for up to 9 years. en_US
dc.language.iso en en_US
dc.title A novel xylene-free deparaffinization method for the extraction of proteins from human derived formalin-fixed paraffin embedded (FFPE) archival tissue blocks en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SOM en_US
dc.author.idnumber 200902750 en_US
dc.author.idnumber 200904962 en_US
dc.author.woa N/A en_US
dc.author.department N/A en_US
dc.description.embargo N/A en_US
dc.relation.journal MethodsX en_US
dc.journal.volume 1 en_US
dc.article.pages 90-95 en_US
dc.keywords Pathology en_US
dc.keywords Paraffin blocks en_US
dc.keywords Protein extraction en_US
dc.keywords Western blot en_US
dc.keywords Xylene en_US
dc.keywords Water en_US
dc.identifier.doi http://dx.doi.org/10.1016/j.mex.2014.07.006 en_US
dc.identifier.ctation Mansour, A., Chatila, R., Bejjani, N., Dagher, C., & Faour, W. H. (2014). A novel xylene-free deparaffinization method for the extraction of proteins from human derived formalin-fixed paraffin embedded (FFPE) archival tissue blocks. MethodsX, 1, 90-95. en_US
dc.author.email rajaa.chatila@lau.edu.lb
dc.author.email wissam.faour@lau.edu.lb en_US
dc.identifier.url http://www.sciencedirect.com/science/article/pii/S2215016114200355
dc.orcid.id https://orcid.org/0000-0002-0120-2275
dc.orcid.id https://orcid.org/0000-0002-0746-3687 en_US


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