Kefir induces cell-cycle arrest and apoptosis in HTLV-1-negative malignant T-lymphocytes

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dc.contributor.author Rizk, Sandra
dc.contributor.author Maalouf, Katia
dc.contributor.author Baydoun, Elias
dc.date.accessioned 2015-10-27T06:59:09Z
dc.date.available 2015-10-27T06:59:09Z
dc.date.copyright 2011
dc.date.issued 2015-10-27
dc.identifier.issn 1179-1322 en_US
dc.identifier.uri http://hdl.handle.net/10725/2349
dc.description.abstract Background: Adult lymphoblastic leukemia (ALL) is a malignancy that occurs in white blood cells. The overall cure rate in children is 85%, whereas it is only 40% in adults. Kefir is an important probiotic that contains many bioactive ingredients, which give it unique health benefits. It has been shown to control several cellular types of cancer. Purpose: The present study investigates the effect of a cell-free fraction of kefir on CEM and Jurkat cells, which are human T-lymphotropic virus type I (HTLV-1)-negative malignant T-lymphocytes. Methods: Cells were incubated with different kefir concentrations. The cytotoxicity of the compound was evaluated by determining the percentage viability of cells. The effect of all the noncytotoxic concentrations of kefir on the proliferation of CEM and Jurkat cells was then assessed. The levels of transforming growth factor-alpha (TGF-α), transforming growth factor- beta1 (TGF-β1), matrix metalloproteinase-2 (MMP-2), and MMP-9 mRNA upon kefir treatment were then analyzed using reverse transcriptase polymerase chain reaction (RT-PCR). Finally, the growth inhibitory effects of kefir on cell-cycle progression/apoptosis were assessed by Cell Death Detection (ELISA) and flow cytometry. Results: The maximum cytotoxicity recorded after 48-hours treatment with 80 μg/μL kefir was only 42% and 39% in CEM and Jurkat cells, respectively. The percent reduction in proliferation was very significant, and was dose-, and time-dependent. In both cell lines, kefir exhibited its antiproliferative effect by downregulating TGF-α and upregulating TGF-β1 mRNA expression. Upon kefir treatment, a marked increase in cell-cycle distribution was noted in the preG1 phase of CEM and Jurkat cells, indicating the proapoptotic effect of kefir, which was further confirmed by Cell Death Detection ELISA. However, kefir did not affect the mRNA expression of metalloproteinases needed for the invasion of leukemic cell lines. Conclusion: In conclusion, kefir is effective in inhibiting proliferation and inducing apoptosis of HTLV-1-negative malignant T-lymphocytes. Therefore, further in vivo investigation is highly recommended. en_US
dc.language.iso en en_US
dc.title Kefir induces cell-cycle arrest and apoptosis in HTLV-1-negative malignant T-lymphocytes en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 199829370 en_US
dc.author.woa N/A en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Cancer management and research en_US
dc.journal.volume 3 en_US
dc.article.pages 39-47 en_US
dc.keywords Apoptosis en_US
dc.keywords Cancer en_US
dc.keywords CEM en_US
dc.keywords Jurkat en_US
dc.keywords Kefir en_US
dc.keywords Leukemia en_US
dc.identifier.doi http://dx.doi.org/10.2147/CMR.S15109 en_US
dc.identifier.ctation Maalouf, K., Baydoun, E., & Rizk, S. (2011). Kefir induces cell-cycle arrest and apoptosis in HTLV-1-negative malignant T-lymphocytes. Cancer management and research, 3, 39. en_US
dc.author.email sandra.rizk@lau.edu.lb
dc.identifier.url http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3064404/

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