The Antiproliferative Effect of Kefir Cell-Free Fraction on HuT-102 Malignant T Lymphocytes

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dc.contributor.author Rizk, Sandra
dc.contributor.author Baydoun, Elias
dc.contributor.author Maalouf, Katia
dc.date.accessioned 2015-10-26T13:31:12Z
dc.date.available 2015-10-26T13:31:12Z
dc.date.copyright 2009
dc.date.issued 2015-10-26
dc.identifier.issn 2152-2650 en_US
dc.identifier.uri http://hdl.handle.net/10725/2345
dc.description.abstract Kefir is produced by adding kefir grains (a mass of proteins, polysaccharides, bacteria, and yeast) to pasteurized milk; it has been shown to control several cellular types of cancer, such as Sarcoma 180 in mice, Lewis lung carcinoma, and human mammary cancer. Human T-cell lymphotropic virus type 1 (HTLV-1) is the causative agent of adult T-cell leukemia, which is a fatal disease with no effective treatment. The current study aims at investigating the effect of a cell-free fraction of kefir on HuT-102 cells, which are HTLV-1–positive malignant T-lymphocytes. Cells were incubated with different kefir concentrations: the cytotoxicity of the compound was evaluated by determining the percentage viability of cells. The effect of all the noncytotoxic concentrations of kefir cell-free fraction on the proliferation of HuT-102 cells was then assessed. The levels of transforming growth factor (TGF)-α mRNA upon kefir treatment were then analyzed using reverse transcriptase polymerase chain reaction. Finally, the growth inhibitory effects of kefir on cell cycle progression and/or apoptosis were assessed by flow cytometry. The maximum cytotoxicity recorded at 80 μg/μL for 48 hours was only 43%. The percent reduction in proliferation was very significant, dose and time dependent, and reached 98% upon 60-μg/μL treatment for 24 hours. Kefir cell-free fraction caused the downregulation of TGF-α, which is a cytokine that induces the proliferation and replication of cells. Finally, a marked increase in cell cycle distribution was noted in the pre-G1 phase. In conclusion, kefir is effective in inhibiting proliferation and inducing apoptosis of HTLV-1–positive malignant T-lymphocytes. Therefore, further in vivo investigation is highly recommended. en_US
dc.title The Antiproliferative Effect of Kefir Cell-Free Fraction on HuT-102 Malignant T Lymphocytes
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 199829370
dc.author.woa N/A en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.article.pages 198-203 en_US
dc.identifier.doi http://dx.doi.org/10.3816/CLM.2009.s.012 en_US
dc.identifier.ctation Rizk, S., Maalouf, K., & Baydoun, E. (2009). The antiproliferative effect of kefir cell-free fraction on HuT-102 malignant T lymphocytes. Clinical Lymphoma and Myeloma, 9, S198-S203. en_US
dc.author.email sandra.rizk@lau.edu.lb
dc.identifier.url http://www.sciencedirect.com/science/article/pii/S1557919011703407

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