Protein and pH-dependent Binding of Nascent Pectin and Glucuronarabinoxylan to Xylogucan in Pea

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dc.contributor.author Rizk, Sandra
dc.contributor.author Abdel-Massih, Roula
dc.contributor.author Baydoun, Elias
dc.contributor.author Brett, Christopher
dc.date.accessioned 2015-10-26T11:23:12Z
dc.date.available 2015-10-26T11:23:12Z
dc.date.copyright 2000
dc.date.issued 2015-10-26
dc.identifier.issn 0032-0935 en_US
dc.identifier.uri http://hdl.handle.net/10725/2339
dc.description.abstract  Nascent pectin and glucuronoarabinoxylan, synthesised in vitro by membrane-bound enzymes from etiolated pea (Pisum sativum L.) epicotyls, were found to bind to pea xyloglucan in a pH-dependent manner. The binding was maximum at low pH (3–4), and decreased to almost zero at pH 6. The binding was probably non-covalent and reached saturation within 5 min. Removal of the fucose residues of xyloglucan decreased the degree of binding. Removal by protease of the proteins attached to nascent pectin and glucuronoarabinoxylan greatly reduced the maximum binding and abolished the pH-dependence. The observed binding may be of considerable significance in the process of cell-wall assembly and in the control of cell extension. en_US
dc.language.iso en en_US
dc.title Protein and pH-dependent Binding of Nascent Pectin and Glucuronarabinoxylan to Xylogucan in Pea en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 199829370 en_US
dc.author.woa N/A en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Planta en_US
dc.journal.volume 211 en_US
dc.article.pages 423-429 en_US
dc.keywords Binding (Pectin) en_US
dc.keywords Glucuronoarabnoxylan en_US
dc.keywords Glycoprotein en_US
dc.keywords Pectin en_US
dc.keywords Pisum en_US
dc.keywords Xyloglucan en_US
dc.identifier.doi http://dx.doi.org/10.1007/s004250000303
dc.identifier.ctation Rizk, S. E., Abdel-Massih, R. M., Baydoun, E. A. H., & Brett, C. T. (2000). Protein-and pH-dependent binding of nascent pectin and glucuronoarabinoxylan to xyloglucan in pea. Planta, 211(3), 423-429. en_US
dc.author.email sandra.rizk@lau.edu.lb
dc.identifier.url http://link.springer.com/article/10.1007/s004250000303

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