Growth hormone treatment modulates active ghrelin levels in rats

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dc.contributor.author Haddad, Haytham
dc.contributor.author Mroueh, Mohamad
dc.contributor.author Faour, Wissam H.
dc.contributor.author Daher, Costantine
dc.date.accessioned 2015-10-23T09:07:18Z
dc.date.available 2015-10-23T09:07:18Z
dc.date.copyright 2014
dc.date.issued 2015-10-23
dc.identifier.issn 0743-5800 en_US
dc.identifier.uri http://hdl.handle.net/10725/2323
dc.description.abstract Introduction: Impairments in neuroendocrine regulation of food intake and postprandial satiety are leading causes to obesity. Ghrelin peptide is a GI hormone known to increase food intake partly through induction of growth hormone. The regulation of ghrelin production is still unknown. Objective: The aim of the current study is to investigate the influence of growth hormone (Genotropin) treatment on active ghrelin levels in plasma, stomach, pancreas and kidney in rats. Material/methods: Male Sprague-Dawley rats, randomly allocated into control and three treatment groups, received daily subcutaneous injections of Genotropin at 2, 20 and 100 µg/rat/day for 5 consecutive days. Active ghrelin levels were quantified per tissue mass or tissue protein. Results: In control groups, the highest active ghrelin concentration in pmol/g tissue was found in the stomach (15.5 ± 0.21) followed by the pancreas (1.96 ± 0.066) and the kidney (1.36 ± 0.037). Genotropin treatment caused a dose dependent decrease in active ghrelin concentration in stomach, kidney and pancreas with a concomitant increase in plasma, and reaching significance at 20 and 100 µg/rat/day doses. However, the treatment caused variable effect on total protein concentrations, with a decrease in pancreas and an increase in stomach and kidney supernatants. Consequently, under such treatment, determination of active ghrelin concentration per tissue mass rather than per tissue protein is favored. Conclusions: The present study suggests a direct correlation between Genotropin treatment and active ghrelin secretion into the rat plasma via modulating its stores in stomach, kidney and pancreas. en_US
dc.language.iso en en_US
dc.title Growth hormone treatment modulates active ghrelin levels in rats en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SOP en_US
dc.author.school SOM
dc.author.school SAS
dc.author.idnumber 199590020
dc.author.idnumber 200904962
dc.author.idnumber 199190130
dc.author.woa N/A en_US
dc.author.department Pharmaceutical Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Endocrine Research en_US
dc.journal.volume 39 en_US
dc.journal.issue 1 en_US
dc.article.pages 39-43 en_US
dc.keywords ELISA en_US
dc.keywords Growth hormone en_US
dc.keywords Kidney en_US
dc.keywords Obesity en_US
dc.keywords Stomach en_US
dc.keywords Pancreas en_US
dc.keywords Ghrelin en_US
dc.identifier.doi http://dx.doi.org/10.3109/07435800.2013.799484 en_US
dc.identifier.ctation Haddad, H., Mroueh, M., Faour, W. H., & Daher, C. (2014). Growth hormone treatment modulates active ghrelin levels in rats. Endocrine research, 39(1), 39-43. en_US
dc.author.email mmroueh@lau.edu.lb
dc.author.email wissam.faour@lau.edu.lb
dc.author.email cdaher@lau.edu.lb
dc.identifier.url http://www.tandfonline.com/doi/abs/10.3109/07435800.2013.799484
dc.orcid.id https://orcid.org/0000-0003-1572-7133 en_US
dc.orcid.id https://orcid.org/0000-0002-8275-7263 en_US

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