Identification and characterization of an ascorbic acid transporter in human granulosa–lutein cells

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dc.contributor.author Zreik, Tony
dc.contributor.author Kodaman, Pinar
dc.contributor.author Jones, Ervin
dc.contributor.author Olive, David
dc.contributor.author Behrman, Harold
dc.date.accessioned 2015-10-07T06:07:42Z
dc.date.available 2015-10-07T06:07:42Z
dc.date.copyright 1999
dc.date.issued 2015-10-07
dc.identifier.issn 1360-9947 en_US
dc.identifier.uri http://hdl.handle.net/10725/2266
dc.description.abstract Ascorbic acid serves a vital role as a pre-eminent antioxidant. In animals, it has been shown to be concentrated in granulosa and theca cells of the follicle, in luteal cells of the corpus luteum, and in the peripheral cytoplasm of the oocyte. We have previously identified hormonally-regulated ascorbic acid transporters in rat granulosa and luteal cells, and herein present preliminary evidence for the presence of a transporter for ascorbic acid in human granulosa–lutein cells. Granulosa–lutein cells were obtained from the follicular fluid of patients undergoing in-vitro fertilization. Following an overnight incubation, the cells were incubated with [14C]-ascorbic acid (0.15 μCi; 150 μMM) and ascorbic acid uptake was determined. The uptake of ascorbic acid was saturable with a Michaeli's constant (Km) and maximum velocity (Vmax) of 21 μM and 3 pmol/106 cells/min respectively. Ouabain, low Na+ medium, and dinitrophenol significantly inhibited ascorbic acid uptake (P < 0.05). Neither the presence of insulin, human chorionic gonadotrophin (HCG), insulin-like growth factor (IGF)-I, nor IGF-II affected the uptake of ascorbic acid in a statistically significant fashion. Following saturation of cellular uptake, the ascorbic acid level was estimated to be 1.04 pmoles/106 cells or ~1 mM, a high concentration similar to that seen in rat luteal cells. Active ascorbic acid transport in human granulosa–lutein cells appears to occur via a Na+- and energy-dependent transporter, with high levels of ascorbic acid being accumulated in these cells. en_US
dc.language.iso en en_US
dc.title Identification and characterization of an ascorbic acid transporter in human granulosa–lutein cells en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SOM en_US
dc.author.idnumber 200802707
dc.author.woa N/A en_US
dc.author.department N/A en_US
dc.description.embargo N/A en_US
dc.relation.journal Molecular human reproduction, en_US
dc.journal.volume 5 en_US
dc.journal.issue 4 en_US
dc.article.pages 299-302 en_US
dc.keywords Ascorbic acid transporter en_US
dc.keywords Human ovary en_US
dc.identifier.doi http://dx.doi.org/10.1093/molehr/5.4.29 en_US
dc.identifier.ctation Zreik, T. G., Kodaman, P. H., Jones, E. E., Olive, D. L., & Behrman, H. (1999). Identification and characterization of an ascorbic acid transporter in human granulosa–lutein cells. Molecular human reproduction, 5(4), 299-302. en_US
dc.author.email tgzreik@lau.edu.lb
dc.identifier.url http://molehr.oxfordjournals.org/content/5/4/299.short

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