Characterisation of Pga1, a putative Candida albicans cell wall protein necessary for proper adhesion and biofilm formation

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dc.contributor.author Malouf, Katia
dc.contributor.author Dimassi, Hani I.
dc.contributor.author Khalaf, Roy A.
dc.contributor.author Hashash, Rami
dc.contributor.author Younes, Samer
dc.contributor.author Bahnan, Wael
dc.contributor.author El-Koussa, Joseph
dc.date.accessioned 2015-09-30T08:23:14Z
dc.date.available 2015-09-30T08:23:14Z
dc.date.issued 2015-09-30
dc.identifier.issn 1439-0507 en_US
dc.identifier.uri http://hdl.handle.net/10725/2211
dc.description.abstract The fungal pathogen Candida albicans is a leading causative agent of death in immunocompromised individuals. Many factors have been implicated in virulence including filamentation-inducing transcription factors, adhesins, lipases and proteases. Many of these factors are glycosylphosphatidylinositol-anchored cell surface antigenic determinant proteins. Pga1 is one such protein shown to be upregulated during cell wall regeneration. The purpose of this study was to characterise the role Pga1 plays by creating a homozygous pga1 null strain and comparing the phenotype with the parental strain. It was observed that the mutant strain showed less oxidative stress tolerance and an increased susceptibility to calcofluor white, a cell surface disrupting agent that inhibits chitin fibre assembly which translated as a 40% decrease in cell wall chitin content. Furthermore, the mutant exhibited a 50% reduction in adhesion and a 33% reduction in biofilm formation compared with the parental strain, which was reflected as a slight reduction in virulence. Our data suggest that Pga1 plays an important role in cell wall rigidity and stability. It was also observed that the pga1 null was over filamentous on both liquid and solid media and exhibited increased resistance to SDS suggesting upregulation of filamentation-inducing genes and cell surface components to partially compensate for the deletion. en_US
dc.language.iso en en_US
dc.title Characterisation of Pga1, a putative Candida albicans cell wall protein necessary for proper adhesion and biofilm formation en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SOP en_US
dc.author.school SAS
dc.author.idnumber 200103543
dc.author.idnumber 200603781
dc.author.idnumber 200300427
dc.author.woa N/A en_US
dc.author.department Pharmacy en_US
dc.description.embargo N/A en_US
dc.relation.journal Mycoses en_US
dc.journal.volume 54 en_US
dc.journal.issue 6 en_US
dc.article.pages 491-500 en_US
dc.keywords Candida albicans en_US
dc.keywords GPI anchor en_US
dc.keywords Adhesion en_US
dc.keywords Chitin en_US
dc.keywords Calcofluo en_US
dc.keywords Congo red en_US
dc.identifier.doi http://dx.doi.org/10.1111/j.1439-0507.2010.01883.x en_US
dc.identifier.ctation Hashash, R., Younes, S., Bahnan, W., El Koussa, J., Maalouf, K., Dimassi, H. I., & Khalaf, R. A. (2011). Characterisation of Pga1, a putative Candida albicans cell wall protein necessary for proper adhesion and biofilm formation. Mycoses, 54(6), 491-500. en_US
dc.author.email katia.maalouf@lau.edu.lb
dc.author.email hani.dimassi@lau.edu.lb
dc.author.email roy.khalaf@lau.edu.lb
dc.identifier.url http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0507.2010.01883.x/full
dc.orcid.id https://orcid.org/0000-0003-1827-2453
dc.orcid.id https://orcid.org/0000-0002-5402-7730

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