The anti-cancer activity of 2 himachalene-6-OL extracted from daucus carota SSP. carota. (c2014)

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dc.contributor.author Najm, Paul I.
dc.date.accessioned 2015-02-11T10:45:34Z
dc.date.available 2015-02-11T10:45:34Z
dc.date.issued 2015-02-11
dc.date.submitted 2014-05-29
dc.identifier.uri http://hdl.handle.net/10725/1937
dc.description Includes bibliographical references (leaves 43-48). en_US
dc.description.abstract Daucus carota ssp. carota, known as wild carrot, is a member of the family Umbelliferae (Apiacae), and commonly used in the folklore medicine in Lebanon to treat several diseases including cancer. Its oil extract and fractions exhibited antioxidant, anti-inflammatory and anti-cancer activities. Daucus carota oil extract was chromatographed on silica gel column and produced four fractions: pentane (F1), 50:50 pentane:diethyl ether (F2), diethyl ether (F3), 93:7 chloroform:methanol (F4) fractions. The four fractions were tested against A549, Caco-2, MB-MDA-231, B16F-10, and SF- 268 cancer cells and F2 demonstrated the highest activity. The major compound of F2 was isolated and identified by GC-MS and NMR spectroscopy as 2-himachalene-6-ol, and found to be unique to the Lebanese Daucus carota. 2 himachalene-6-ol showed promising anti-proliferative and anti-metastatic abilities against the used cells, with high sensitivity against SF-268 cells. Staining with Annexin V and PI, using flow cytometry, showed that 2 himachalene-6-ol treatment induced cellular death through necrosis. Moreover, time lapse movies and wound healing assays demonstrated that 2 himachalene-6-ol decreased 2D cellular motility in SF-268 cells in vitro. Adhesion and 3D invasion assays done also showed that the treatment decreased SF-268 cellular adhesion and invasion on collagen in vitro. The significance of the study is that 2 himachalene-6-ol could be considered towards reaching a potential cancer therapy targeting proliferation and apoptosis, as well as cancer motility and metastasis. en_US
dc.language.iso en en_US
dc.subject Carrots -- Therapeutic use en_US
dc.subject Cancer cells -- Proliferation en_US
dc.subject Metastasis -- Prevention en_US
dc.subject Cancer -- Alternative treatment en_US
dc.subject Dissertations, Academic en_US
dc.subject Lebanese American University -- Dissertations en_US
dc.title The anti-cancer activity of 2 himachalene-6-OL extracted from daucus carota SSP. carota. (c2014) en_US
dc.type Thesis en_US
dc.term.submitted Spring en_US
dc.author.degree MS in Molecular Biology en_US
dc.author.school Arts and Sciences en_US
dc.author.idnumber 201105109 en_US
dc.author.commembers Dr. Costantine Daher
dc.author.commembers Dr. Mohamad Mroueh
dc.author.woa OA en_US
dc.description.physdesc 1 hard copy: xiv, 48 leaves; ill. (some col.); 30 cm. available at RNL. en_US
dc.author.division Biology en_US
dc.author.advisor Dr. Mirvat El Sibai
dc.keywords Dacaus carota en_US
dc.keywords Cancer en_US
dc.keywords Proliferation en_US
dc.keywords Apoptosis en_US
dc.keywords Metastasis en_US
dc.keywords Motility en_US
dc.identifier.doi https://doi.org/10.26756/th.2014.19 en_US
dc.publisher.institution Lebanese American University en_US

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