A comparative study of the antiproliferative effect of kohlrabi and green cabbage on colorectal cancer cell lines. (c2012)

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dc.contributor.author Zarzour, Valia M.
dc.date.accessioned 2013-02-27T09:47:33Z
dc.date.available 2013-02-27T09:47:33Z
dc.date.copyright 2012 en_US
dc.date.issued 2013-02-27
dc.date.submitted 2012-09-24
dc.identifier.uri http://hdl.handle.net/10725/1479
dc.description Includes bibliographical references (leaves 52-62). en_US
dc.description.abstract Experimental studies showed that phytochemicals from Brassicaceae Cruciferae vegetables possess anticarcinogenic properties. This association is attributed to glucosinolates molecules, mainly isothiocyanates and indoles released upon consumption of these vegetables. Two members of this family, namely cabbage (Brassica oleracea, group Capitata) and kohlrabi (Brassica oleracea, group Gongylodes) seem to share similar anticarcinogenic characteristics. The aim of this study is to compare the effect of the ethanolic extracts of the edible parts of these two plants on the proliferation and apoptosis of HT-29 and Caco-2 colorectal cancer cell lines. The cytotoxicity of the extracts was assayed using the Trypan Blue Exclusion method and the IC50 was determined to be higher in kohlrabi compared to cabbage. Consequently, WST-1 assay was used to assess the effect of these plant extracts on the proliferation of colorectal cancer cell lines: both extracts exhibited a sharp antiproliferative effect in a dose and time dependent manner, even though the cell viability recorded upon kohlrabi treatment was slightly higher. Analysis of cell DNA content by flow cytomtery revealed the ability of both kohlrabi and cabbage extracts to induce cell cycle arrest in HT-29 cells at the G phase and S phase in similar patterns. Furthermore both extracts promoted apoptosis in colorectal cancer cell lines, as revealed by Cell Death ELISA Assay, as well as Annexin/PI staining followed by flow cytometry analysis. The apoptotic pathway seems to involve the pro-apoptotic protein Bax and the anti-apoptotic protein Bcl-2: the expression of these apoptotic regulatory proteins was altered upon cabbage and kohlrabi treatments in a dose-dependent manner, with a slightly higher increase in the Bax/Bcl-2 ratio upon treatment with cabbage extract. As a conclusion, kohlrabi should be considered, like cabbage, among the potent anti-carcinogenic cruciferous vegetables. en_US
dc.language.iso en en_US
dc.subject Materia medica, Vegetable en_US
dc.subject Cancer cells -- Proliferation en_US
dc.subject Colon (Anatomy) -- Cancer -- Treatment en_US
dc.title A comparative study of the antiproliferative effect of kohlrabi and green cabbage on colorectal cancer cell lines. (c2012) en_US
dc.type Thesis en_US
dc.term.submitted Summer II en_US
dc.author.degree MS in Molecular Biology en_US
dc.author.school Arts and Sciences en_US
dc.author.idnumber 200503272 en_US
dc.author.commembers Dr. Roy Khalaf
dc.author.commembers Dr. Sima Tokajian
dc.author.woa OA en_US
dc.description.physdesc 1 bound copy: xvi, 62 leaves; ill.; 31 cm. available at RNL. en_US
dc.author.division Biology en_US
dc.author.advisor Dr. Sandra Rizk
dc.keywords Kohlrabi en_US
dc.keywords Colorectal cancer en_US
dc.keywords Proliferation en_US
dc.keywords Apoptosis en_US
dc.keywords DIM en_US
dc.keywords SFN en_US
dc.keywords Erucin en_US
dc.keywords AITC en_US
dc.keywords Bax en_US
dc.keywords Bcl-2 en_US
dc.identifier.doi https://doi.org/10.26756/th.2012.32 en_US
dc.publisher.institution Lebanese American University en_US

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