Phenotypic and Genotypic Characteristics of Clinical Pseudomonas aeruginosa Isolates

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dc.contributor.author Timane, Hind
dc.date.accessioned 2023-03-13T09:48:18Z
dc.date.available 2023-03-13T09:48:18Z
dc.date.copyright 2022 en_US
dc.date.issued 2022-11-30
dc.identifier.uri http://hdl.handle.net/10725/14556
dc.description.abstract Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen associated with human nosocomial infections. It causes chronic infections in pulmonary passages, as in cystic fibrosis patients, urinary tract infections, and sepsis. It is a ubiquitous bacterium found in animals, plants, and soil. Multi-drug resistant P. aeruginosa is recognized by the World Health Organization (WHO) as an important public health concern. In this study, whole genome sequencing (WGS) based analysis of nine P. aeruginosa, one Pseudomonas stutzeri, and one Pseudomonas putida isolates was performed to understand their molecular epidemiology. Genome comparison, multi- locus sequence typing (MLST), and whole genome SNP- based phylogenetic analysis (wgSNP) were used to determine the relatedness of the isolates. In silico typing was used to determine the antimicrobial resistance, virulence determinants, and plasmids. PCR was used to detect exoS and exoU genes, and biofilm assays were performed to identify biofilm formers. Nine distinct MLST allelic profiles were identified. Antimicrobial susceptibility tests showed that all the isolates were susceptible to kanamycin, while six isolates were resistant to piperacillin-tazobactam. Genomic analysis revealed the presence of several β-lactamases (blaPAO, blaOXA-50, blaPAU-1, and blaVIM-2) and aminoglycoside resistance encoding determinants (aph(3’)-IIb, aac(6’)-29a, and aac(2’)-Ia). exoU and exoS toxin encoding genes were detected in 54% and 45% of the isolates, respectively, and 63% of the isolates were biofilm formers. Six different plasmids were detected, and a number of virulence factors related to toxins, kinases, and pigments that contribute to the ability of the bacterium to adapt to environmental changes. Our study revealed the diversity among P. aeruginosa in Lebanese hospitals. The emergence and spread of MDR P. aeruginosa emphasize on the importance of integrating whole-genome sequencing into infection control and outbreak detection. en_US
dc.language.iso en en_US
dc.subject Pseudomonas aeruginosa en_US
dc.subject Pseudomonas aeruginosa infections -- Pathogenesis en_US
dc.subject Molecular epidemiology en_US
dc.subject Lebanese American University -- Dissertations en_US
dc.subject Dissertations, Academic en_US
dc.title Phenotypic and Genotypic Characteristics of Clinical Pseudomonas aeruginosa Isolates en_US
dc.type Thesis en_US
dc.title.subtitle Antimicrobial Susceptibility Profiles and Molecular Typing en_US
dc.term.submitted Fall en_US
dc.author.degree Doctor of Pharmacy en_US
dc.author.school SAS en_US
dc.author.idnumber 201600944 en_US
dc.author.commembers Khalaf, Roy
dc.author.commembers Wex, Brigitte
dc.author.department Natural Sciences en_US
dc.description.physdesc 1 online resource (xiii, 67 leaves): col. ill. en_US
dc.author.advisor Tokajian, Sima
dc.keywords Pseudomonas aeruginosa en_US
dc.keywords Molecular epidemiology en_US
dc.keywords Whole-genome sequencing en_US
dc.keywords Clonal relatedness,Multi-drug Resistance en_US
dc.description.bibliographiccitations Includes bibliographical references (leaves 42-67). en_US
dc.identifier.doi https://doi.org/10.26756/th.2022.514
dc.author.email hind.timane@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php en_US
dc.publisher.institution Lebanese American University en_US
dc.author.affiliation Lebanese American University en_US

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