Transfusion of Plasma from Young Exercise mice ameliorates Aging-associated Cognitive Impairments on Learning and Memory through activation of Autophagy

LAUR Repository

Show simple item record

dc.contributor.author Khoury, Reine
dc.date.accessioned 2022-10-26T07:06:54Z
dc.date.available 2022-10-26T07:06:54Z
dc.date.copyright 2022 en_US
dc.date.issued 2022-06-18
dc.identifier.uri http://hdl.handle.net/10725/14130
dc.description.abstract The brain’s cognitive skills gradually decline with aging. In old animals, damaged proteins accumulate in neurons since autophagy, a catabolic process responsible for organelle and protein degradation, decreases. Physical exercise is a known lifestyle factor that promotes learning and memory formation in the hippocampus. The beneficial effects of exercise are mediated through the induction of the brain derived neurotrophic factor (BDNF). Previous work identified that exercise promotes cognition by inducing autophagy. In this study, we report that voluntary exercise increases autophagic activity in the hippocampus of adult C57BL/6 mice. This increase in autophagy is correlated with enhanced spatial learning and memory formation in the Morris water maze. Inhibition of autophagy in adult exercise mice with chloroquine phosphate (CQ) during the behavioral test showed impaired learning and memory formation, as well as decreased BDNF levels in the hippocampus as compared to the control exercise group. Activation of BDNF signaling in mice treated with CQ did not rescue learning and memory deficits. Hence, our results suggest that BDNF signaling is upstream of autophagy in the hippocampus. The same exercise paradigm did not promote learning and memory formation in middle aged and old male mice. Interestingly, we show that systemic administration of adult exercise plasma into middle-aged mice rejuvenates learning and memory in an autophagy dependent manner. Our results are consistent with autophagy playing central roles in promoting exercise-induced effects on cognition. Among the plasma factors, we identified β-hydroxybutyrate, a liver-derived molecule, as an exercise-induced factor that promotes learning and memory in an autophagy-dependent manner. The results reveal the potential therapeutic benefits of plasma factors released in response to exercise in an autophagy dependent manner. en_US
dc.language.iso en en_US
dc.subject Autophagic vacuoles -- Therapeutic use en_US
dc.subject Hippocampus (Brain) -- Physiology en_US
dc.subject Brain -- Diseases -- Prevention en_US
dc.subject Neurotrophic functions en_US
dc.subject Exercise therapy en_US
dc.subject Lebanese American University -- Dissertations en_US
dc.subject Dissertations, Academic en_US
dc.title Transfusion of Plasma from Young Exercise mice ameliorates Aging-associated Cognitive Impairments on Learning and Memory through activation of Autophagy en_US
dc.type Thesis en_US
dc.term.submitted Summer en_US
dc.author.degree Doctor of Pharmacy en_US
dc.author.school SAS en_US
dc.author.idnumber 201704733 en_US
dc.author.commembers Rizk, Sandra
dc.author.commembers Daher, Costantine
dc.author.department Natural Sciences en_US
dc.description.physdesc 1 online resource (xii, 58 leaves): ill. (some col.) en_US
dc.author.advisor Sleiman, Sama
dc.author.advisor Stephan, Joseph
dc.keywords Aging en_US
dc.keywords Autophagy en_US
dc.keywords BDNF en_US
dc.keywords Brain en_US
dc.keywords Learning en_US
dc.keywords LC3B en_US
dc.keywords Memory en_US
dc.keywords Plasma factors en_US
dc.keywords Voluntary exercise en_US
dc.description.bibliographiccitations Includes bibliographical references (leaves 45-58). en_US
dc.identifier.doi https://doi.org/10.26756/th.2022.458
dc.author.email reine.elkhoury@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php en_US
dc.publisher.institution Lebanese American University en_US
dc.author.affiliation Lebanese American University en_US

Files in this item

This item appears in the following Collection(s)

Show simple item record

Search LAUR

Advanced Search


My Account