Whole Genome Molecular Characterization of Methicillin Resistant Staphylococcus aureus Isolated From Clinical Settings in Lebanon

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dc.contributor.author Almachtoub, Tia
dc.date.accessioned 2022-08-18T08:11:49Z
dc.date.available 2022-08-18T08:11:49Z
dc.date.copyright 2022 en_US
dc.date.issued 2022-05-16
dc.identifier.uri http://hdl.handle.net/10725/13949
dc.description.abstract The high prevalence of multi-drug resistant Staphylococcus aureus, particularly the Methicillin-Resistant S. aureus (MRSA), has become a worldwide health concern. In this study, we used whole-genome sequencing, multi-locus Sequence Typing (MLST), S. aureus protein A (spa), and Staphylococcal Chromosomal Cassette (SCCmec) typing of MRSA and pan-genome analysis to study the molecular characteristics, resistance patterns, and relatedness of the isolates. In silico typing was used to determine the antimicrobial resistance and virulence determinants, rep families, and prophages. All the studied isolates carried the blaZ gene, and most were multi-drug resistant. Two MRSA isolates recovered from hospitalized patients were additionally resistant to vancomycin. Seven distinct MLST allelic profiles were identified, and the most common STs and spa types, respectively, were ST789 and ST1and t091 and t127. SCCmec typing of MRSA revealed the prevalence of type V(5C2). MRSA ST789-t091-V was the predominant type, but we also detected SCCmec cassette harboring ccrA1B1 and mecC2, classified as SCCmec type IX(1C2). en_US
dc.language.iso en en_US
dc.subject Staphylococcus aureus infections -- Lebanon en_US
dc.subject Methicillin resistance -- Lebanon en_US
dc.subject Lebanese American University -- Dissertations en_US
dc.subject Dissertations, Academic en_US
dc.title Whole Genome Molecular Characterization of Methicillin Resistant Staphylococcus aureus Isolated From Clinical Settings in Lebanon en_US
dc.type Thesis en_US
dc.term.submitted Spring en_US
dc.author.degree Doctor of Pharmacy en_US
dc.author.school SAS en_US
dc.author.idnumber 201406321 en_US
dc.author.commembers Khalaf, Roy
dc.author.commembers Khalil, Christian
dc.author.department Natural Sciences en_US
dc.description.physdesc 1 online resource (xiii, 70 leaves): col. ill. en_US
dc.author.advisor Tokajian, Sima
dc.keywords Staphylococcus aureus en_US
dc.keywords WGS en_US
dc.keywords MLST en_US
dc.keywords SCCmec en_US
dc.keywords spa en_US
dc.keywords Lebanon en_US
dc.description.bibliographiccitations Includes bibliographical references (leaf 53-70). en_US
dc.identifier.doi https://doi.org/10.26756/th.2022.431
dc.author.email tia.almashtoub@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php en_US
dc.publisher.institution Lebanese American University en_US
dc.author.affiliation Lebanese American University en_US

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