Sternbergia Clusiana Bulb Ethanolic Extracts Induce Apoptosis in MCF-7 Breast Cancer Cells

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dc.contributor.author Elias, Nazira
dc.date.accessioned 2022-08-12T09:02:51Z
dc.date.available 2022-08-12T09:02:51Z
dc.date.copyright 2022 en_US
dc.date.issued 2022-05-05
dc.identifier.uri http://hdl.handle.net/10725/13927
dc.description.abstract Sternbergia is a genus of plants belonging to the Amaryllidaceae family which is a popular ornamental plant. Plant leaf and bulb extracts of various Sternbergia species have been proven to possess diverse significant biological effects and have been historically employed in traditional medicine. However, the anti-tumor effects of Sternbergia clusiana bulbs in particular has not been investigated to date. The present study explores the anti-cancer activity of the ethanolic extract of Sternbergia clusiana bulbs (SbBEE) on breast cancer MCF-7 cell line in vitro and deciphers the underlying molecular mechanism associated with it. Treatment of MCF-7 cells with SbBEE showed a dose- and time-dependent anti-proliferative effect along with the occurrence of numerous apoptotic hallmarks. A dose-dependent increase in apoptosis via induced DNA fragmentation was revealed via Cell Death ELISA as well as an increase in cellular fragmentation using flow cytometry. On a molecular level, western blot analysis showed an upregulation of pro-apoptotic proteins such as poly(ADP-ribose) polymerase (PARP), Bax/Bcl-2 ratio and caspase-9, with no alterations in caspase-8 levels, revealing the activation of the intrinsic apoptotic pathway. Moreover, SbBEE exposure caused a significant reduction in ROS production proposing a potent anti-oxidant potential. A number of chemical compounds that contributed to SbBEE’s pro-apoptotic activity on MCF-7 cells were detected by liquid chromatography coupled with mass spectrometry, including lycorine, 9S,13R-12-Oxophytodienoic acid (OPDA), fumaritine N-oxide, rhamnetin and ferulic acid. The data presented in this study reveal promising anti-cancerous effects of SbBEE on MCF-7 breast cancer cells in vitro that should be further evaluated in vivo. en_US
dc.language.iso en en_US
dc.subject Breast -- Cancer -- Molecular aspects en_US
dc.subject Breast -- Cancer -- Treatment en_US
dc.subject Apoptosis en_US
dc.subject Cancer cells -- Proliferation en_US
dc.subject Lebanese American University -- Dissertations en_US
dc.subject Dissertations, Academic en_US
dc.title Sternbergia Clusiana Bulb Ethanolic Extracts Induce Apoptosis in MCF-7 Breast Cancer Cells en_US
dc.type Thesis en_US
dc.title.subtitle Molecular Mechanisms and Chemical Characterization en_US
dc.term.submitted Spring en_US
dc.author.degree Doctor of Pharmacy en_US
dc.author.school SAS en_US
dc.author.idnumber 201508562 en_US
dc.author.commembers Houri, Ahmad
dc.author.commembers Khalil, Christian
dc.author.department Natural Sciences en_US
dc.description.physdesc 1 online resource (xii, 65 leaves): ill. (some col.) en_US
dc.author.advisor Rizk-Jamati, Sandra
dc.keywords Sternbergia Clusiana en_US
dc.keywords ROS en_US
dc.keywords MCF-7 Cells en_US
dc.keywords Medicinal Plants en_US
dc.keywords Breast Cancer en_US
dc.keywords Apoptosis en_US
dc.description.bibliographiccitations Includes bibliographical references (leaf 49-65) en_US
dc.identifier.doi https://doi.org/10.26756/th.2022.413
dc.author.email nazira.elias@lau.edu en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php en_US
dc.publisher.institution Lebanese American University en_US
dc.author.affiliation Lebanese American University en_US

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