Human skin explants an in vitro approach for assessing UVB induced damage

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dc.contributor.author Khalil, Christian
dc.date.accessioned 2022-07-29T11:01:18Z
dc.date.available 2022-07-29T11:01:18Z
dc.date.copyright 2018 en_US
dc.date.issued 2022-07-29
dc.identifier.issn 0887-2333 en_US
dc.identifier.uri http://hdl.handle.net/10725/13895
dc.description.abstract Lifestyle changes involving frequent outdoor activities are contributing to higher exposure to harmful ultraviolet light (UVB). The acute effects of UVB irradiation on human skin was evaluated in this study using freshly excised human skin from elective surgery subjected to UVB doses (0–3.76 J/cm2). The assessment of UVB induced cellular and skin damages was undertaken at two time points immediately and 24 h post exposure using in vitro, and immunohistochemical staining techniques. The results indicated no significant loss of skin integrity or significant acute mitochondrial cellular damages in UVB exposed skin sections as measured by the MTS cytotoxicity assay. The other key markers of damage showed significant extracellular LDH membrane leakages and upregulation of inflammatory cytokines such as IL-1β. Skin integrity analysis was also undertaken using H&E, HLADR, and anti-cytokeratin antibodies. The results showed significant epidermal changes, basal cell activation and Langerhans cells depletion. The research proved the usefulness of freshly excised human skin explant model in measuring UVB damage. Furthermore, freshly excised human skin maintains the natural layering and therefore does not pose the same challenges faced by commercially available reconstructed skin in terms of higher costs and accurate mimicking of all the complex interactions observed in human skin. en_US
dc.language.iso en en_US
dc.title Human skin explants an in vitro approach for assessing UVB induced damage en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 201408580 en_US
dc.author.department Natural Sciences en_US
dc.relation.journal Toxicology in Vitro en_US
dc.journal.volume 53 en_US
dc.article.pages 193-199 en_US
dc.keywords UVB en_US
dc.keywords Skin en_US
dc.keywords HLADR en_US
dc.keywords MTS en_US
dc.keywords H&e en_US
dc.keywords Cytokeratin en_US
dc.keywords IL-1β en_US
dc.keywords Langerhans cells en_US
dc.identifier.doi https://doi.org/10.1016/j.tiv.2018.08.013 en_US
dc.identifier.ctation Khalil, C. (2018). Human skin explants an in vitro approach for assessing UVB induced damage. Toxicology in Vitro, 53, 193-199. en_US
dc.author.email christian.khalil@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url https://www.sciencedirect.com/science/article/pii/S0887233318304958 en_US
dc.orcid.id https://orcid.org/0000-0002-9782-1870 en_US
dc.author.affiliation Lebanese American University en_US

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