The Effect of DJ-1 on Invasion and Invadopodia Formation in Glioblastoma Multiforme

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dc.contributor.author Ezzeddine, Aya
dc.date.accessioned 2022-07-21T09:15:07Z
dc.date.available 2022-07-21T09:15:07Z
dc.date.copyright 2020 en_US
dc.date.issued 2020-05-05
dc.identifier.uri http://hdl.handle.net/10725/13849
dc.description.abstract Glioblastoma multiform (GBM) is one of the most aggressive malignant brain tumors. Advances in the treatment of this cancer are limited by the infiltrating nature of the GBM cancer cells. DJ-1 is an oncogene which stimulates cell proliferation by inhibiting the tumor suppressor PTEN and leads to the subsequent activation of the PI3Kinase pathway. We have shown in the lab that DJ-1 knockdown decreases 2D cell motility of breast cancer cells. In this study, we sought to investigate the role of DJ-1 in 3D migration and invasion and particularly, in the formation of invadopodia. To this aim, we first investigated the various proteins that DJ-1 interacts with and the subsequent pathways they modulate to better understand its role in cancer. Then, we investigated how DJ-1 affects cell viability and the PI3K/PTEN pathway in glioblastoma cells. We next examined the expression and subcellular localization of DJ-1 and noted that it localizes at the invadopodia sites. Consistently, co-immunofluorescence of DJ-1 with structural proteins which regulate actin dynamics, namely, ARP2 and SH3PXD2B demonstrated a clear co-localization of DJ-1 with both markers at the invadopodia. Our results also revealed that the number of invadopodia decreases upon DJ-1 depletion. en_US
dc.language.iso en en_US
dc.subject Glioblastoma multiforme en_US
dc.subject Cancer invasiveness -- Molecular aspects en_US
dc.subject Cancer -- Molecular aspects en_US
dc.subject Lebanese American University -- Dissertations en_US
dc.subject Dissertations, Academic en_US
dc.title The Effect of DJ-1 on Invasion and Invadopodia Formation in Glioblastoma Multiforme en_US
dc.type Thesis en_US
dc.term.submitted Spring en_US
dc.author.degree MS in Molecular Biology en_US
dc.author.school SAS en_US
dc.author.idnumber 201400475 en_US
dc.author.commembers Khalaf, Roy
dc.author.commembers Ghassibe-Sabbagh, Michella
dc.author.department Natural Sciences en_US
dc.description.physdesc 1 online resource (xv, 78 leaves): col. ill. en_US
dc.author.advisor El Sibai, Mirvat
dc.keywords Glioblastoma multiform en_US
dc.keywords DJ-1 en_US
dc.keywords Invadopodia en_US
dc.keywords PI3K pathway en_US
dc.keywords Actin dynamics en_US
dc.description.bibliographiccitations Includes bibliographical references (leaf 54-78). en_US
dc.identifier.doi https://doi.org/10.26756/th.2022.375
dc.author.email aya.ezzeddine@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php en_US
dc.publisher.institution Lebanese American University en_US
dc.author.affiliation Lebanese American University en_US

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