The Effect of HuArgI (Co)-PEG5000 on The Migration of Ovarian Cancer

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dc.contributor.author El Mais, Nour
dc.date.accessioned 2022-07-20T08:43:07Z
dc.date.available 2022-07-20T08:43:07Z
dc.date.copyright 2020 en_US
dc.date.issued 2020-05-22
dc.identifier.uri http://hdl.handle.net/10725/13842
dc.description.abstract Ovarian cancer being the first cause of death in gynecological diseases, paves the way towards increasing the search for a targeted therapy that could control the metastasis of a late-stage diagnosed ovarian cancer. HuArgI (Co)-PEG5000 is a potential drug to target cancers that depend on exogenous sources of arginine and cannot synthesize their own. In this study, we hypothesized that HuArgI (Co)- PEG5000 can decrease the migration of ovarian cancer cells. Our results show a decrease in the migratory rate of the cells and their rate in wound closure. The second thing we looked into was the adhesion of those cells and the formation of focal adhesions. The number and area of focal adhesions were quantified and the results show a decrease in the area and number of focal adhesions upon treating with HuArgI (Co)-PEG5000. Finally, we looked at the effect of HuArgI (Co)-PEG5000 on 3D motility of the ovarian cancer cell line, using a collagen based assay. It showed that SKOV3 cell line does not show significant invasiveness to collagen. In conclusion, we noticed an effect on ovarian cancer by treating the cells with HuArgI (Co)-PEG5000 which opens the way for further studies on the effect of arginine deprivation and autophagy induction on the migration of ovarian cancer. en_US
dc.language.iso en en_US
dc.subject Ovaries -- Cancer -- Treatment en_US
dc.subject Cancer cells -- Motility en_US
dc.subject Arginine -- Therapeutic use en_US
dc.subject Lebanese American University -- Dissertations en_US
dc.subject Dissertations, Academic en_US
dc.title The Effect of HuArgI (Co)-PEG5000 on The Migration of Ovarian Cancer en_US
dc.type Thesis en_US
dc.term.submitted Spring en_US
dc.author.degree Doctor of Pharmacy en_US
dc.author.school SAS en_US
dc.author.idnumber 201502076 en_US
dc.author.commembers Khalaf, Roy
dc.author.commembers Ghassibe-Sabbagh, Michella
dc.author.department Natural Sciences en_US
dc.description.physdesc 1 online resource (xi, 60 leaves): col. ill. en_US
dc.author.advisor El Sibai, Mirvat
dc.author.advisor Abi Habib, Ralph
dc.keywords HuArgI (Co)-PEG5000 en_US
dc.keywords Arginine en_US
dc.keywords Migration en_US
dc.keywords Autophagy en_US
dc.keywords Ovarian Cancer en_US
dc.keywords SKOV3 en_US
dc.description.bibliographiccitations Bibliography: leaf 48-50. en_US
dc.identifier.doi https://doi.org/10.26756/th.2022.368
dc.author.email nour.elmays@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php en_US
dc.publisher.institution Lebanese American University en_US
dc.author.affiliation Lebanese American University en_US

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