Phenotypic and Genotypic Typing of Carbapenem-Resistant Acinetobacter baumannii Isolates from Lebanon

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dc.contributor.author Sleiman Haidar, Siwar
dc.date.accessioned 2022-06-16T08:35:13Z
dc.date.available 2022-06-16T08:35:13Z
dc.date.copyright 2021 en_US
dc.date.issued 2021-05-28
dc.identifier.uri http://hdl.handle.net/10725/13713
dc.description.abstract Infections caused by Carbapenem-Resistant Acinetobacter baumannii (CRAB) have become an essential healthcare-associated problem. We used whole-genome sequencing (WGS) to characterize A. baumannii collected from a hospital in Lebanon. The genotypic relatedness was assessed by pulsed-field gel electrophoresis (PFGE), multi-locus sequence typing (MLST), and whole-genome SNP-based phylogenetic analysis (wg-SNP). A. baumannii PCR-based replicon typing (AB-PBRT) was also used to analyze the plasmid content. PFGE demonstrated that most isolates that belonged to a unique clonal type were assigned to ST2 of the international clone II. Phenotypic antimicrobial susceptibility testing revealed that the isolates were extensively drug-resistant (XDR). Intrinsic β-lactam resistance genes, including blaADC25 (Acinetobacter-Derived Cephalosporinase) and the blaOXA66 (a variant of blaOXA51), were among the common resistance determinants. AB-PBRT, which categorizes A. baumannii plasmids into homology groups (GRs) based on the nucleotide homology of their respective replicase genes, showed that the isolates were mainly positive for GR2 and GR6. A. baumannii is a nosocomial pathogen that is difficult to control. WGS analysis showed the relatedness of the studied CRAB isolates and mobile genetic elements and revealed the enhanced transmission prospects of A. baumannii between and among patients. WGS and interventional trials are needed to define and prevent the spread of XDR microbial pathogens. en_US
dc.language.iso en en_US
dc.subject Acinetobacter en_US
dc.subject Acinetobacter infections en_US
dc.subject Bacteria -- Analysis en_US
dc.subject Lebanese American University -- Dissertations en_US
dc.subject Dissertations, Academic en_US
dc.title Phenotypic and Genotypic Typing of Carbapenem-Resistant Acinetobacter baumannii Isolates from Lebanon en_US
dc.type Thesis en_US
dc.term.submitted Spring en_US
dc.author.degree Doctor of Pharmacy en_US
dc.author.school SAS en_US
dc.author.idnumber 201300755 en_US
dc.author.commembers Wex, Brigitte
dc.author.commembers Khalaf, Roy
dc.author.department Natural Sciences en_US
dc.description.physdesc 1 online resource (xii, 60 leaves): ill. (some col.) en_US
dc.author.advisor Tokajian, Sima
dc.keywords Acinetobacter baumannii en_US
dc.keywords CRAB en_US
dc.keywords WGS en_US
dc.keywords PFGE en_US
dc.keywords Outbreak en_US
dc.description.bibliographiccitations Includes bibliographical references (leaf 45-60) en_US
dc.identifier.doi https://doi.org/10.26756/th.2022.201
dc.author.email siwar.sleimanhaidar@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php en_US
dc.publisher.institution Lebanese American University en_US
dc.author.affiliation Lebanese American University en_US

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