dc.contributor.author |
El Samarji, Mona |
|
dc.date.accessioned |
2022-06-16T05:50:14Z |
|
dc.date.available |
2022-06-16T05:50:14Z |
|
dc.date.copyright |
2021 |
en_US |
dc.date.issued |
2021-04-29 |
|
dc.identifier.uri |
http://hdl.handle.net/10725/13689 |
|
dc.description.abstract |
Extensive research has revealed that Amaryllidaceae species exhibit numerous therapeutic properties through several mechanisms. Among the various Amaryllidaceae species, Sternbergia clusiana has been recently given a great amount of attention in the medicinal field due to its richness in small chemical molecules, namely Amaryllidaceae alkaloids; these have been reported to possess an antioxidant and anti-inflammatory role, in addition to a prominent anti-cancer effect on different types of cancer cell lines. The current study investigates the selective anti-proliferative effect of Sternbergia clusiana bulb ethanolic extract (SbBEE) on breast cancer cells cultured in vitro (MDA-MB-231 and MCF-7) and reveals the underlying mechanism responsible for the induction of cell death. MDA-MB-231 cells treated with SbBEE exhibited morphological and biochemical hallmarks of apoptosis that were detected through DNA fragmentation, the hyper-expression of phosphatidylserine on the outer leaflet of cells membrane and the upregulation of proteins able to mediate cell death through apoptosis (c-PARP, Bax/ Bcl2). The prominent decrease in ROS levels upon treatment confirmed the antioxidant property of the extract being investigated, highlighting the promising effect of SbBEE in breast cancer treatment. |
en_US |
dc.language.iso |
en |
en_US |
dc.subject |
Breast -- Cancer -- Molecular aspects |
en_US |
dc.subject |
Breast -- Cancer -- Treatment |
en_US |
dc.subject |
Apoptosis |
en_US |
dc.subject |
Cancer cells -- Proliferation |
en_US |
dc.subject |
Lebanese American University -- Dissertations |
en_US |
dc.subject |
Dissertations, Academic |
en_US |
dc.title |
The Antioxidant and Pro-apoptotic effects of Sternbergia clusiana Bulb Ethanolic Extract on Breast Cancer Cells in vitro |
en_US |
dc.type |
Thesis |
en_US |
dc.term.submitted |
Spring |
en_US |
dc.author.degree |
Doctor of Pharmacy |
en_US |
dc.author.school |
SAS |
en_US |
dc.author.idnumber |
201502138 |
en_US |
dc.author.commembers |
Houri, Ahmad |
|
dc.author.commembers |
Khalil, Christian |
|
dc.author.department |
Natural Sciences |
en_US |
dc.description.physdesc |
1 online resource (xi, 57 leaves): ill. (some col.) |
en_US |
dc.author.advisor |
Rizk-Jamati, Sandra |
|
dc.keywords |
Sternbergia clusiana |
en_US |
dc.keywords |
Breast cancer |
en_US |
dc.keywords |
MDA-MB-231 |
en_US |
dc.keywords |
MCF-7 |
en_US |
dc.keywords |
Apoptosis |
en_US |
dc.description.bibliographiccitations |
Bibliography: leaf 50-57 |
en_US |
dc.identifier.doi |
https://doi.org/10.26756/th.2022.225 |
|
dc.identifier.tou |
http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php |
en_US |
dc.publisher.institution |
Lebanese American University |
en_US |
dc.author.affiliation |
Lebanese American University |
en_US |