The Antioxidant and Pro-apoptotic effects of Sternbergia clusiana Bulb Ethanolic Extract on Breast Cancer Cells in vitro

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dc.contributor.author El Samarji, Mona
dc.date.accessioned 2022-06-16T05:50:14Z
dc.date.available 2022-06-16T05:50:14Z
dc.date.copyright 2021 en_US
dc.date.issued 2021-04-29
dc.identifier.uri http://hdl.handle.net/10725/13689
dc.description.abstract Extensive research has revealed that Amaryllidaceae species exhibit numerous therapeutic properties through several mechanisms. Among the various Amaryllidaceae species, Sternbergia clusiana has been recently given a great amount of attention in the medicinal field due to its richness in small chemical molecules, namely Amaryllidaceae alkaloids; these have been reported to possess an antioxidant and anti-inflammatory role, in addition to a prominent anti-cancer effect on different types of cancer cell lines. The current study investigates the selective anti-proliferative effect of Sternbergia clusiana bulb ethanolic extract (SbBEE) on breast cancer cells cultured in vitro (MDA-MB-231 and MCF-7) and reveals the underlying mechanism responsible for the induction of cell death. MDA-MB-231 cells treated with SbBEE exhibited morphological and biochemical hallmarks of apoptosis that were detected through DNA fragmentation, the hyper-expression of phosphatidylserine on the outer leaflet of cells membrane and the upregulation of proteins able to mediate cell death through apoptosis (c-PARP, Bax/ Bcl2). The prominent decrease in ROS levels upon treatment confirmed the antioxidant property of the extract being investigated, highlighting the promising effect of SbBEE in breast cancer treatment. en_US
dc.language.iso en en_US
dc.subject Breast -- Cancer -- Molecular aspects en_US
dc.subject Breast -- Cancer -- Treatment en_US
dc.subject Apoptosis en_US
dc.subject Cancer cells -- Proliferation en_US
dc.subject Lebanese American University -- Dissertations en_US
dc.subject Dissertations, Academic en_US
dc.title The Antioxidant and Pro-apoptotic effects of Sternbergia clusiana Bulb Ethanolic Extract on Breast Cancer Cells in vitro en_US
dc.type Thesis en_US
dc.term.submitted Spring en_US
dc.author.degree Doctor of Pharmacy en_US
dc.author.school SAS en_US
dc.author.idnumber 201502138 en_US
dc.author.commembers Houri, Ahmad
dc.author.commembers Khalil, Christian
dc.author.department Natural Sciences en_US
dc.description.physdesc 1 online resource (xi, 57 leaves): ill. (some col.) en_US
dc.author.advisor Rizk-Jamati, Sandra
dc.keywords Sternbergia clusiana en_US
dc.keywords Breast cancer en_US
dc.keywords MDA-MB-231 en_US
dc.keywords MCF-7 en_US
dc.keywords Apoptosis en_US
dc.description.bibliographiccitations Bibliography: leaf 50-57 en_US
dc.identifier.doi https://doi.org/10.26756/th.2022.225
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php en_US
dc.publisher.institution Lebanese American University en_US
dc.author.affiliation Lebanese American University en_US

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