Phenotypic and Molecular Characterization of Multi-drug Resistant Klebsiella spp. Isolates Recovered from Clinical Settings

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dc.contributor.author Diab, Hassan
dc.date.accessioned 2022-06-14T09:08:22Z
dc.date.available 2022-06-14T09:08:22Z
dc.date.copyright 2021 en_US
dc.date.issued 2021-07-27
dc.identifier.uri http://hdl.handle.net/10725/13657
dc.description.abstract Klebsiella pneumoniae is a Gram-negative bacterium that colonizes the gastrointestinal tract and nasopharynx with the vast majority associated with hospitalization and causing human nosocomial infections. Extended-spectrum β-lactamases (ESBL)-producing and carbapenem-resistant K. pneumoniae is recognized by the World Health Organization (WHO) as a serious public health concern. In this study, whole-genome sequencing (WGS) – based analysis was performed to understand the molecular epidemiology of multi-drug resistant Klebsiella spp. clinical isolates. Genome comparison, multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and whole-genome-SNP-based phylogenetic analysis (wg-SNP) were used to determine the relatedness of the isolates. in silico typing was used to determine the resistance genes, virulence factors, Inc groups, and capsular types. All except one isolate were non-susceptible to meropenem and 89% were non-susceptible to ertapenem and imipenem. blaNDM, blaOXA-48, and blaKPC were the carbapenemase-linked genes with blaNDM-1 being detected in half of the sequenced isolates. Resistance to colistin was detected in one and was correlated to mutations in ccrB, pmrB, and pmrC genes. The most common plasmid type was IncFIB followed by IncR, and the Type 3 fimbriae, chromosomally encoded by the mrkABCDF operon, was conserved among the sequenced Klebsiella spp. The most common sequence (ST) and K-type detected were ST147 and K64. A nosocomial outbreak linked to a carbapenem-resistant ST147 K. pneumoniae supported by SNPs-based analysis, PFGE clustering, and genome comparison was also detected. This study provides crucial insights into antimicrobial resistance and the virulence of an increasingly important global pathogen. It also confirms that whole-genome sequencing data should be integrated into routine and targeted pathogen surveillance, outbreak detection, and infection control. en_US
dc.language.iso en en_US
dc.subject Klebsiella pneumoniae en_US
dc.subject Klebsiella quasipneumoniae en_US
dc.subject Drug resistance in microorganisms en_US
dc.subject Medical microbiology en_US
dc.subject Lebanese American University -- Dissertations en_US
dc.subject Dissertations, Academic en_US
dc.title Phenotypic and Molecular Characterization of Multi-drug Resistant Klebsiella spp. Isolates Recovered from Clinical Settings en_US
dc.type Thesis en_US
dc.term.submitted Summer en_US
dc.author.degree Doctor of Pharmacy en_US
dc.author.school SAS en_US
dc.author.idnumber 201605569 en_US
dc.author.commembers Wex, Brigitte
dc.author.commembers Khalaf, Roy
dc.author.department Natural Sciences en_US
dc.description.physdesc 1 online resource (xiii, 71 leaves): col. ill. en_US
dc.author.advisor Tokajian, Sima
dc.keywords Klebsiella pneumoniae. en_US
dc.keywords Klebsiella quasipneumoniae en_US
dc.keywords Klebsiella variicola en_US
dc.keywords ST147 en_US
dc.keywords Outbreak en_US
dc.keywords NDM en_US
dc.keywords OXA-48 en_US
dc.keywords KPC-2 en_US
dc.description.bibliographiccitations Includes bibliographical references (leaf 53-71) en_US
dc.identifier.doi https://doi.org/10.26756/th.2022.254
dc.author.email hassan.diab01@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php en_US
dc.publisher.institution Lebanese American University en_US
dc.author.affiliation Lebanese American University en_US

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