Palladin Regulates Invadopodia Formation in Glioblastoma

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dc.contributor.author Abi Khalil, Elie
dc.date.accessioned 2022-06-14T05:38:26Z
dc.date.available 2022-06-14T05:38:26Z
dc.date.copyright 2021 en_US
dc.date.issued 2021-07-23
dc.identifier.uri http://hdl.handle.net/10725/13644
dc.description.abstract Glioblastoma multiforme (GBM), also referred to as glioblastoma is a rapidly dividing glioma that develops from astrocytes and oligodendrocytes (glial cells that nourish the brain). GBM can also be classified as a grade IV astrocytoma, which represents the most invasive, rapidly growing, and metastatic type of glial tumors. Our lab has been primarily interested in identifying and studying pathways relevant to the progression of this disease through which we recently determined that hypoxia and EGF stimulation contribute to VEGF expression via the differential regulation of the PI3K/Rho-GTPase and MAPK signaling pathways. Palladin is a relatively novel actin-associated phosphoprotein that has been reported to be implicated in a variety of cancers such as pancreatic adenocarcinoma and ductal carcinoma of the breast. It has also been reportedly involved in the development of invadopodia, dorsal ruffles, podosomes, focal adhesions, and has been shown to be involved in regulating the expression of the Rho-GTPases, Cdc42 and Rac1. More recently, palladin has been shown to alter actin dynamics in podocytes as well as replace the Arp2/3 complex during Listeria infections, contributing to the assembly of actin-based structures. In this study, we used a palladin knockdown model in order to assess invadopodia formation in glioblastoma cell line U87. The results show an increase in invadopodia as seen by TKS4 and TKS5 staining as well as a decrease in Cdc42 activity following palladin knockdown. We have also been able to demonstrate a novel role for palladin in the expression of Rac1. This study serves to characterize palladin within the context of invadopodia formation and as such we have been able to identify potential crosstalk between palladin and Rho GTPases such as Cdc42 and Rac1. en_US
dc.language.iso en en_US
dc.subject Rho GTPases en_US
dc.subject Cell division en_US
dc.subject Glioblastoma multiforme en_US
dc.subject Cancer cells en_US
dc.subject Lebanese American University -- Dissertations en_US
dc.subject Dissertations, Academic en_US
dc.title Palladin Regulates Invadopodia Formation in Glioblastoma en_US
dc.type Thesis en_US
dc.term.submitted Summer en_US
dc.author.degree Doctor of Pharmacy en_US
dc.author.school SAS en_US
dc.author.idnumber 201600160 en_US
dc.author.commembers Georgess, Dan
dc.author.commembers Ghassibe-Sabbagh, Michella
dc.author.department Natural Sciences en_US
dc.description.physdesc 1 online resource (xiii, 46 leaves) : col. ill. en_US
dc.author.advisor El Sibai, Mirvat
dc.keywords Glioblastoma en_US
dc.keywords Palladin en_US
dc.keywords Cell invasion en_US
dc.keywords Invadopodia en_US
dc.keywords Rho GTPases en_US
dc.keywords Cdc42 en_US
dc.keywords RhoC en_US
dc.keywords Rac1 en_US
dc.description.bibliographiccitations Includes bibliographical references (leaf 36-46) en_US
dc.identifier.doi https://doi.org/10.26756/th.2022.360
dc.author.email elie.abikhalil@lau.edu en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php en_US
dc.publisher.institution Lebanese American University en_US
dc.author.affiliation Lebanese American University en_US

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