Characterizing the effect of arginine deprivation on breast cancer cell migration and proliferation

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dc.contributor.author Fayad, Chantal
dc.date.accessioned 2022-06-13T10:59:50Z
dc.date.available 2022-06-13T10:59:50Z
dc.date.copyright 2021 en_US
dc.date.issued 2021-12-27
dc.identifier.uri http://hdl.handle.net/10725/13642
dc.description.abstract Depriving cells of arginine, a semi-essential amino acid, is a developing strategy to target various cancer types. However, little is known about the effect of arginine-degrading enzymes in breast cancer, a disease of diverse molecular and phenotypic subtypes. In this project, we utilized a pharmacologically formulated human recombinant arginase (HuArgI(Co)-PEG5000) to evaluate the impact of arginine deprivation on breast cancer. We studied three breast cancer cell lines MCF-7 (a non-metastatic model for luminal breast cancer), UACC-2087 (a slow-growing model of triple negative breast cancer), and MDA-MB-231 (a highly aggressive and metastatic model of triple negative breast cancer). Using wound healing assay, Ki67 immunofluorescence assay, and annexin v pi assay, we respectively found that a 72-hour HuArgI(Co)-PEG5000 treatment mitigated MCF-7 and UACC-2087 cells’ migration, proliferation and survival. Interestingly, while treatment of MDA-MB-231 for a shorter time frame (24h) with HuArgI(Co)-PEG5000 did not result in robust anti-migratory or anti-proliferative effects, longer (72h) incubations induced a level of cell death in this cell line superseding that of UACC-2087 and MDA-MB-231. Our findings suggest the presence of differential mechanisms of action for arginine depriving enzymes across breast cancer subtypes, and point towards robust cytotoxic effects in highly proliferative triple negative breast cancer cells. en_US
dc.language.iso en en_US
dc.subject Arginine -- Therapeutic use en_US
dc.subject Breast -- Cancer -- Prevention en_US
dc.subject Cancer cells -- Motility en_US
dc.subject Lebanese American University -- Dissertations en_US
dc.subject Dissertations, Academic en_US
dc.title Characterizing the effect of arginine deprivation on breast cancer cell migration and proliferation en_US
dc.type Thesis en_US
dc.term.submitted Fall en_US
dc.author.degree Doctor of Pharmacy en_US
dc.author.school SAS en_US
dc.author.idnumber 201905938 en_US
dc.author.commembers Ghassibe-Sabbagh, Michella
dc.author.commembers Abi-Habib, Ralph
dc.author.department N/A en_US
dc.description.physdesc 1 online resource (xi, 57 leaves) : coll. ill. en_US
dc.author.advisor Georgess, Dan
dc.keywords Breast Cancer en_US
dc.keywords Arginine en_US
dc.keywords Arginase en_US
dc.keywords Migration en_US
dc.keywords Proliferation en_US
dc.keywords Survival en_US
dc.description.bibliographiccitations Bibliography: leaf 38-57. en_US
dc.identifier.doi https://doi.org/10.26756/th.2022.358
dc.author.email chantal.fayad@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php en_US
dc.publisher.institution Lebanese American University en_US
dc.author.affiliation Lebanese American University en_US

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