Annona cherimola Seed Extract Activates Extrinsic and Intrinsic Apoptotic Pathways in Leukemic Cells By Peter Nasr A thesis

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dc.contributor.author Nasr, Peter
dc.date.accessioned 2022-04-27T09:39:14Z
dc.date.available 2022-04-27T09:39:14Z
dc.date.copyright 2020 en_US
dc.date.issued 2020-05-05
dc.identifier.uri http://hdl.handle.net/10725/13518
dc.description.abstract Annona cherimola Mill is a large green fruit with black seeds widely known to possess toxic properties due to the presence of Annonaceous acetogenins. The present study investigates the anti-cancer properties of an A. cherimola Mill ethanolic seed extract on Acute Myeloid Leukemia (AML) cell lines in vitro and elucidates the underlying cellular mechanism. The antiproliferative effects of the extract on various AML cell lines and normal mesenchymal cells (MSCs) were assessed using WST-1 viability reagent. The pro-apoptotic effect of the extract was evaluated using Annexin V/PI staining and Cell Death ELISA. The underlying mechanism was deciphered by analyzing the expression of various proteins using western blots. Treatment with an A. cherimola seed ethanolic extract promotes a dose- and time-dependent inhibition of the proliferation of various AML cell lines, but not MSCs. Positive Annexin V staining, as well as DNA fragmentation, confirm an increase in apoptotic cell death by upregulating the expression of pro-apoptotic proteins which control both intrinsic and extrinsic pathways of apoptosis. GC/MS analysis revealed the presence of phytosterols, in addition to other bioactive compounds. In conclusion, A. cherimola seed extract, previously known to possess a potent toxic activity, induces apoptosis in AML cell lines by the activation of both the extrinsic and the intrinsic pathways. en_US
dc.language.iso en en_US
dc.subject Cherimoya -- Therapeutic use en_US
dc.subject Cancer cells -- Proliferation en_US
dc.subject Acute myeloid leukemia -- Treatment en_US
dc.subject Apoptosis -- Molecular aspects en_US
dc.subject Lebanese American University -- Dissertations en_US
dc.subject Dissertations, Academic en_US
dc.title Annona cherimola Seed Extract Activates Extrinsic and Intrinsic Apoptotic Pathways in Leukemic Cells By Peter Nasr A thesis en_US
dc.type Thesis en_US
dc.term.submitted Spring en_US
dc.author.degree Doctor of Pharmacy en_US
dc.author.school SAS en_US
dc.author.idnumber 201404225 en_US
dc.author.commembers Tokajian, Sima
dc.author.commembers Daher, Costantine
dc.author.department N/A en_US
dc.description.physdesc 1 online resource (xi, 50 leaves) : ill. (some col.) en_US
dc.author.advisor Rizk, Sandra
dc.keywords Annona cherimola en_US
dc.keywords Leukemia en_US
dc.keywords Cancer en_US
dc.keywords Apoptosis en_US
dc.keywords Phytosterols en_US
dc.description.bibliographiccitations Includes bibliographical references (leaf 40-50) en_US
dc.identifier.doi https://doi.org/10.26756/th.2022.286
dc.author.email peter.nasr@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php en_US
dc.publisher.institution Lebanese American University en_US
dc.author.affiliation Lebanese American University en_US

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