16S rRNA Gene-Based Characterization and Functional Profiling of the Microbiome in Young Adults

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dc.contributor.author Makhlouf, Rita
dc.date.accessioned 2022-04-12T08:44:03Z
dc.date.available 2022-04-12T08:44:03Z
dc.date.copyright 2020 en_US
dc.date.issued 2020-07-29
dc.identifier.uri http://hdl.handle.net/10725/13479
dc.description.abstract The human gut microbiota is a diverse consortium of organisms inhabiting the gut and playing an essential role in individual health. Analyzing the gut microbial composition of healthy individuals is crucial to set up a baseline for recognizing microbial dysbiosis in the case of a disorder. We aimed to characterize the microbiome of healthy young Lebanese adults through 16S rRNA gene sequencing and analysis. Forty-eight fecal samples were collected from donors in the age range of 18-25. Results obtained revealed that the dominant phyla were Firmicutes (F) and Bacteroidetes (B), followed by Proteobacteria and Tenericutes. The main common predicted metabolic functions were linked to carbohydrate metabolism and transporters. Significant segregation was observed between the microbiome of individuals with C-section versus vaginal delivery records. The microbiome, in the latter being less diverse. This study is the first of its type revealing the gut microbiome profile in young Lebanese adults. It showed a high interindividual variability despite the homogenous characteristics of the subjects. The mean F/B obtained was 1.84, falling between the mean European and the rural Africans. The high observed Firmicutes to Bacteroidetes ratio and the dominance of Bacteroides over Prevotella are associated with a diet rich in fat and proteins. An increased Proteobacteria level was linked with gut dysbiosis and reported in Type 2 diabetes, metabolic disorders, and Inflammatory Bowel Disease (IBD). This study's findings indicated the genetic heterogeneity of the donors and the diversity in Lebanese eating habits. en_US
dc.language.iso en en_US
dc.subject Human body -- Microbiology en_US
dc.subject Young adults -- Diseases -- Prevention en_US
dc.subject Bacteroides en_US
dc.subject Bacterial diversity -- Genetic aspects en_US
dc.subject Lebanese American University -- Dissertations en_US
dc.subject Dissertations, Academic en_US
dc.title 16S rRNA Gene-Based Characterization and Functional Profiling of the Microbiome in Young Adults en_US
dc.type Thesis en_US
dc.term.submitted Spring en_US
dc.author.degree Doctor of Pharmacy en_US
dc.author.school SAS en_US
dc.author.idnumber 201400335 en_US
dc.author.commembers Khalaf, Roy
dc.author.commembers Akoury, Elias
dc.author.department Natural Sciences en_US
dc.description.physdesc 1 online resource (xiv, 79 leaves) : col. ill. en_US
dc.author.advisor Tokajian, Sima
dc.keywords Microbiome en_US
dc.keywords 16S rRNA en_US
dc.keywords Lebanon en_US
dc.keywords QIIME2 en_US
dc.keywords Bacteroidetes en_US
dc.keywords Firmicutes en_US
dc.keywords Actinobacteria en_US
dc.keywords Proteobacteria en_US
dc.keywords BURRITO en_US
dc.keywords C-section delivery en_US
dc.description.bibliographiccitations Includes bibliographical references (leaf 70-79). en_US
dc.identifier.doi https://doi.org/10.26756/th.2022.307
dc.author.email rita.makhlouf@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php en_US
dc.publisher.institution Lebanese American University en_US
dc.author.affiliation Lebanese American University en_US

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