Cardiac phosphodiesterases are differentially increased in diabetic cardiomyopathy

LAUR Repository

Show simple item record

dc.contributor.author Hanna, Rita
dc.contributor.author Nour-Eldine, Wared
dc.contributor.author Saliba, Youakim
dc.contributor.author Dagher-Hamalian, Carole
dc.contributor.author Hachem, Pia
dc.contributor.author Abou-Khalil, Pamela
dc.contributor.author Mika, Delphine
dc.contributor.author Varin, Audrey
dc.contributor.author El Hayek, Magali Samia
dc.contributor.author Pereira, Laëtitia
dc.contributor.author Fares, Nassim
dc.contributor.author Vandecasteele, Grégoire
dc.contributor.author Abi-Gerges, Aniella
dc.date.accessioned 2021-12-23T13:29:15Z
dc.date.available 2021-12-23T13:29:15Z
dc.date.copyright 2021 en_US
dc.date.issued 2021-12-23
dc.identifier.issn 0024-3205 en_US
dc.identifier.uri http://hdl.handle.net/10725/13185
dc.description.abstract Aim Diabetic cardiomyopathy (DCM) accomodates a spectrum of cardiac abnormalities. This study aims to investigate whether DCM is associated with changes in cyclic adenosine 3′-5′ monophosphate (cAMP) signaling, particularly cyclic nucleotide phosphodiesterases (PDEs). Main methods Type 1 diabetes (T1D) was induced in rats by streptozotocin (STZ, 65 mg/kg) injection. Myocardial remodeling, structure and function were evaluated by histology and echocardiography, respectively. We delineated the sequential changes affecting cAMP signaling and characterized the expression pattern of the predominant cardiac PDE isoforms (PDE 1–5) and β-adrenergic (β-AR) receptors at 4, 8 and 12 weeks following diabetes induction, by real-time quantitative PCR and Western blot. cAMP levels were measured by immunoassays. Key findings T1D-induced DCM was associated with cardiac remodeling, steatosis and fibrosis. Upregulation of β1-AR receptor transcripts was noted in diabetic hearts at 4 weeks along with an increase in cAMP levels and an upregulation in the ejection fraction and fraction shortening. However, β2-AR receptors expression remained unchanged regardless of the disease stage. Moreover, we noted an early and specific upregulation of cardiac PDE1A, PDE2A, PDE4B, PDE4D and PDE5A expression at week 4, followed by increases in PDE3A levels in diabetic hearts at week 8. However, DCM was not associated with changes in PDE4A gene expression irrespective of the disease stage. Significance We show for the first time differential and time-specific regulations in cardiac PDEs, data that may prove useful in proposing new therapeutic approaches in T1D-induced DCM. en_US
dc.language.iso en en_US
dc.title Cardiac phosphodiesterases are differentially increased in diabetic cardiomyopathy en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SOM en_US
dc.author.idnumber 200103210 en_US
dc.author.idnumber 201402416 en_US
dc.author.department N/A en_US
dc.relation.journal Life Sciences en_US
dc.journal.volume 283 en_US
dc.keywords β-Adrenergic receptors en_US
dc.keywords Cyclic adenosine 3′-5′ monophosphate en_US
dc.keywords 3′-5′ cyclic nucleotide phosphodiesterase en_US
dc.keywords Diabetic cardiomyopathy en_US
dc.keywords Type 1 diabetes en_US
dc.identifier.doi https://doi.org/10.1016/j.lfs.2021.119857 en_US
dc.identifier.ctation Hanna, R., Nour-Eldine, W., Saliba, Y., Dagher-Hamalian, C., Hachem, P., Abou-Khalil, P., ... & Abi-Gerges, A. (2021). Cardiac Phosphodiesterases Are Differentially Increased in Diabetic Cardiomyopathy. Life Sciences, 283, 119857. en_US
dc.author.email pamela.aboukhalil@lau.edu.lb en_US
dc.author.email aniella.abigerges@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url https://www.sciencedirect.com/science/article/abs/pii/S0024320521008444 en_US
dc.orcid.id https://orcid.org/0000-0001-9974-4023 en_US
dc.author.affiliation Lebanese American University en_US

Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search LAUR

Advanced Search


My Account