Phenotypic and cell wall proteomic characterization of a DDR48 mutant candida albicans strain

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dc.contributor.author El Khoury, Pamela
dc.contributor.author Salameh, Carell
dc.contributor.author Younes, Samer
dc.contributor.author Awad, Andy
dc.contributor.author Said, Yana
dc.contributor.author Khalaf, Roy A.
dc.date.accessioned 2019-12-10T10:54:30Z
dc.date.available 2019-12-10T10:54:30Z
dc.date.copyright 2019 en_US
dc.date.issued 2019-12-10
dc.identifier.issn 1738-8872 en_US
dc.identifier.uri http://hdl.handle.net/10725/11621
dc.description.abstract Candida albicans is an opportunistic fungus possessing multiple virulence factors controlling pathogenicity. Cell wall proteins are the most important among these factors, being the first elements contacting the host. Ddr48 is a cell wall protein consisting of 212 amino acids. A DDR48 haploinsufficient mutant strain was previously found necessary for proper oxidative stress response and drug resistance. In this study, we aimed to further elucidate the role of Ddr48 by performing additional phenotypic characterization assays. A combinatory proteomic and bioinformatics approach was also undertaken to determine differentially expressed cell wall proteins. Results showed that the mutant strain exhibited a 10% decrease in adhesion mirrored by a 20% decrease in biofilm formation, and slight sensitivity to menadione, diamide, and SDS. Both strains showed similar hyphae formation, virulence, temperature tolerance, and calcofluor white and Congo red sensitivities. Furthermore, a total of 8 and 10 proteins were identified exclusively in the wild-type strain grown under filamentous and nonfilamentous conditions respectively. Results included proteins responsible for superoxide stress resistance (Sod4 and Sod6), adhesion (Als3, Hyr4, Pmt1, and Utr2), biofilm formation (Hsp90, Ece1, Rim9, Ipp1, and Pra1) and cell wall integrity (Utr2 and Pga4). The lack of detection of these proteins in the mutant strain correlates with the observed phenotypes. en_US
dc.language.iso en en_US
dc.title Phenotypic and cell wall proteomic characterization of a DDR48 mutant candida albicans strain en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 200300427 en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Journal of Microbiology and Biotechnology en_US
dc.journal.volume 29 en_US
dc.journal.issue 11 en_US
dc.article.pages 1806-1816 en_US
dc.keywords Candida albicans en_US
dc.keywords Cell wall en_US
dc.keywords Biofilms en_US
dc.keywords Oxidative stress en_US
dc.keywords Tandem mass spectrometry en_US
dc.identifier.doi https://doi.org/10.4014/jmb.1907.07005 en_US
dc.identifier.ctation El, P. K., Salameh, C., Younes, S., Awad, A., Said, Y., & Khalaf, R. (2019). Phenotypic and Cell Wall Proteomic Characterization of a DDR48 mutant Candida albicans Strain. Journal of microbiology and biotechnology 29(11), 1806-1816 en_US
dc.author.email roy.khalaf@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url https://europepmc.org/article/med/31546294 en_US
dc.author.affiliation Lebanese American University en_US

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