Characterization and cytotoxicity assessment of nargile smoke using dynamic exposure

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dc.contributor.author Khalil, Christian
dc.contributor.author Chahine, Joe Braham
dc.contributor.author Chahla, Brenda
dc.contributor.author Hobeika, Tamara
dc.contributor.author Khnayzer, Rony S.
dc.date.accessioned 2019-11-07T13:09:08Z
dc.date.available 2019-11-07T13:09:08Z
dc.date.copyright 2019 en_US
dc.date.issued 2019-11-07
dc.identifier.issn 0895-8378 en_US
dc.identifier.uri http://hdl.handle.net/10725/11504
dc.description.abstract Objectives: Nargile (waterpipe) smoking has gained popularity in the Middle East and throughout the world. In this research, a new dynamic methodology was conceived. This methodology was deployed for direct in vitro assessment of cytotoxicity, genotoxicity, and apoptotic potential of smoke generated from a single nargile session. Materials and methods: A549 cells were deployed in a designed system to assess the cytotoxicity of generated smoke. The smoke was characterized using Gas chromatography–mass spectrometry (GC-MS) profiling for major organic compounds, whereas the remaining chemical and physical parameters were tabulated from published data. The cytoxicity of smoke generated from five commercial flavored tobacco products was assessed using the (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulphophenyl)-2-H-tetrazolium) (MTS) assay. The genotoxicity was also measured using the comet assay, while apopoptosis was evaluated using Annexin V/propidium iodide staining. Results: The data indicated acute cytotoxicity emanating from smoke products in all tested tobacco flavors. Significant loss of viability and mitochondrial activity was observed 40 min post smoke exposure (Double-Apple flavored), while DNA damage onset was reported as early as 20 min of exposure. Microscopical analysis showed a systematic increase in cell rounding post exposure indicating cellular loss of adhesion and potential membrane damages. Finally, the Annexin V/propidium iodide cellular staining showed signs of late apoptosis or necrosis in exposed cells. Conclusions: The presented data clearly indicated significant in vitro cytotoxicity, genotoxicity and apoptosis/necrosis associated with a 60-min single session of nargile smoking. en_US
dc.language.iso en en_US
dc.title Characterization and cytotoxicity assessment of nargile smoke using dynamic exposure en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 201408580 en_US
dc.author.idnumber 200501196 en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Inhalation Toxicology en_US
dc.article.pages 1-14 en_US
dc.keywords Nargile en_US
dc.keywords Smoke en_US
dc.keywords Flavored tobacco en_US
dc.keywords Dynamic method en_US
dc.keywords Cytotoxicity assessment en_US
dc.identifier.doi https://doi.org/10.1080/08958378.2019.1683104 en_US
dc.identifier.ctation Khalil, C., Chahine, J. B., Chahla, B., Hobeika, T., & Khnayzer, R. S. (2019). Characterization and cytotoxicity assessment of nargile smoke using dynamic exposure. Inhalation toxicology, 1-14. en_US
dc.author.email christian.khalil@lau.edu.lb en_US
dc.author.email rony.khnayzer@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url https://www.tandfonline.com/doi/full/10.1080/08958378.2019.1683104 en_US
dc.orcid.id https://orcid.org/0000-0002-9782-1870 en_US
dc.orcid.id https://orcid.org/0000-0001-7775-0027 en_US
dc.author.affiliation Lebanese American University en_US

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